Roy P, Bishop D H, LeBlois H, Erasmus B J
Laboratory of Molecular Biophysics, University of Oxford, UK.
Vaccine. 1994 Jul;12(9):805-11. doi: 10.1016/0264-410x(94)90289-5.
Insect cells co-infected with appropriate recombinant baculoviruses synthesize double-shelled, virus-like particles (VLPs) with bluetongue virus (BTV) VP2 proteins representing serotype 1 (BTV-1), 2 (BTV-2), 10 (BTV-10), 13 (BTV-13) or 17 (BTV-17) as previously reported for BTV-10 (French, T.J., Marshall, J.J.A. and Roy, P. J. Virol. 1990, 64, 5696-5700). The derived particles were purified and used to vaccine sheep, either as single VLP types (BTV-10, BTV-17) or as a combination of all five serotypes. Control sheep received saline. The virus-neutralizing antibody responses were measured. Depending on the experiment, the sheep were challenged with homologous (BTV-10, -13, -17) or selected heterologous (BTV-4, -11, -16) viruses either after 4 months or 14 months, and the disease, viraemias and clinical reactions monitored. The results indicated that two doses of 10 micrograms of VLPs elicited a long-lasting immune response which protected the sheep against challenge with the homologous virulent virus. In certain cases, partial protection was afforded against challenge by heterologous BTV serotypes.
如先前关于BTV - 10的报道（French, T.J., Marshall, J.J.A.和Roy, P. J. Virol. 1990, 64, 5696 - 5700），用合适的重组杆状病毒共感染昆虫细胞，可合成具有代表血清型1（BTV - 1）、2（BTV - 2）、10（BTV - 10）、13（BTV - 13）或17（BTV - 17）的蓝舌病病毒（BTV）VP2蛋白的双层病毒样颗粒（VLP）。将得到的颗粒纯化后用于给绵羊接种疫苗，接种方式为单一VLP类型（BTV - 10、BTV - 17）或五种血清型的组合。对照绵羊接受生理盐水注射。检测病毒中和抗体反应。根据实验情况，在4个月或14个月后，用同源病毒（BTV - 10、 - 13、 - 17）或选定的异源病毒（BTV - 4、 - 11、 - 16）对绵羊进行攻毒，并监测疾病、病毒血症和临床反应。结果表明，两剂10微克的VLP可引发持久的免疫反应，保护绵羊免受同源强毒病毒的攻击。在某些情况下，对异源BTV血清型的攻毒也能提供部分保护。
