Allardet-Servent A, Bourg G, Ramuz M, Pages M, Bellis M, Roizes G
Institut National de la Santé et de la Recherche Médicale, Unité 65, Montpellier-Nîmes, Faculté de Médecine, France.
J Bacteriol. 1988 Oct;170(10):4603-7. doi: 10.1128/jb.170.10.4603-4607.1988.
Preparations of DNA from 23 Brucella strains including 19 reference strains were compared by restriction endonuclease analysis. Pulsed-field gel electrophoresis resulted in optimal resolution of fragments generated by digestion with low-cleavage-frequency restriction enzymes such as XbaI. By this technique, five electrophoretypes were distinguished in five reference strains of the different species, i.e., B. abortus, B. melitensis, B. suis, B. canis, and B. ovis. Minor profile differences allowed us to discriminate between most biovars within a species. However, the differences in the DNA patterns of different field strains of biovar 2 of B. melitensis were not sufficient to serve as markers for epidemiological studies. From the XbaI fragments, we were able to estimate the size of the genomes of B. abortus 544T and B. melitensis 16 MT. This method revealed a relationship between DNA fingerprints, species, and pathovars which could shed light on problems concerning the classification and evolution of members of the genus Brucella.
通过限制性内切酶分析比较了包括19株参考菌株在内的23株布鲁氏菌菌株的DNA制备物。脉冲场凝胶电泳能够最佳地分辨用低切割频率限制性酶（如XbaI）消化产生的片段。通过该技术，在不同种的五个参考菌株中，即流产布鲁氏菌、羊布鲁氏菌、猪布鲁氏菌、犬布鲁氏菌和绵羊布鲁氏菌中区分出了五种电泳型。微小的图谱差异使我们能够区分一个种内的大多数生物变种。然而，羊布鲁氏菌生物变种2不同田间菌株的DNA图谱差异不足以作为流行病学研究的标记。从XbaI片段中，我们能够估计流产布鲁氏菌544T和羊布鲁氏菌16MT的基因组大小。该方法揭示了DNA指纹、种和致病型之间的关系，这可能有助于阐明布鲁氏菌属成员的分类和进化问题。
