Higuchi T, Hayashi H, Abe K
Soy Sauce Research Laboratory, R & D Division of Kikkoman Corporation, Noda City, Chiba, Japan.
J Bacteriol. 1997 May;179(10):3362-4. doi: 10.1128/jb.179.10.3362-3364.1997.
Lactobacillus sp. strain E1 catalyzed the decarboxylation of glutamate (Glu), resulting in a nearly stoichiometric release of the products gamma-aminobutyrate (GABA) and CO2. This decarboxylation was associated with the net synthesis of ATP. ATP synthesis was inhibited almost completely by nigericin and about 70% by N,N'-dicyclohexylcarbodiimide (DCCD), without inhibition of the decarboxylation. These findings are consistent with the possibility that a proton motive force arises from the cytoplasmic proton consumption that accompanies glutamate decarboxylation and the electrogenic Glu/GABA antiporter and the possibility that this proton motive force is coupled with ATP synthesis by DCCD-sensitive ATPase.
乳酸杆菌属菌株E1催化谷氨酸（Glu）的脱羧反应，几乎化学计量地释放出产物γ-氨基丁酸（GABA）和二氧化碳。这种脱羧反应与ATP的净合成相关。尼日利亚菌素几乎完全抑制ATP合成，N,N'-二环己基碳二亚胺（DCCD）抑制约70%，但不抑制脱羧反应。这些发现符合以下可能性：质子动力源自谷氨酸脱羧伴随的细胞质质子消耗以及电生性的Glu/GABA反向转运体；并且这种质子动力通过对DCCD敏感的ATP酶与ATP合成相偶联。
