Ericsson C, Pethö Z, Mehlin H
Karolinska Institutet, Department of Cell and Molecular Biology, Medical Nobel Institute, Stockholm, Sweden.
Electrophoresis. 1997 Mar-Apr;18(3-4):484-90. doi: 10.1002/elps.1150180324.
An annotated two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) protein database of adult Drosophila melanogaster has been constructed, based on the protein patterns of heads, thoraces and abdomens of adult male and female Drosophila melanogaster. About 1200 major protein spots are catalogued. Common proteins, found in all body parts, as well as bodypart- and sex-specifically expressed proteins are reported. Of the major proteins, 91, or 7.5%, are differentially expressed in the two sexes or in different body parts, at least in part reflecting specific functional requirements. At the present time 43 proteins, or about 3.5% of the detected proteins, have been identified. These data can be accessed interactively from our World Wide Web (WWW) server through clickable inline gel images and hypertext links. Identified protein spots are cross-referenced, through hypertext links, to the SWISS-PROT annotated database of protein primary sequences and the Fly-Base database of Drosophila genomic data. Our reference gels can be used to gain immediate access to protein spot identify and to the pattern of differentially expressed proteins in Drosophila melanogaster. The work presented in this article ties together information from protein 2-D PAGE, molecular biology and genetics and offers a uniform way to access this large volume of data.
基于成年雄性和雌性黑腹果蝇头部、胸部和腹部的蛋白质图谱，构建了一个带有注释的成年黑腹果蝇二维聚丙烯酰胺凝胶电泳（2-D PAGE）蛋白质数据库。共记录了约1200个主要蛋白质斑点。报告了在所有身体部位都能找到的常见蛋白质，以及身体部位和性别特异性表达的蛋白质。在主要蛋白质中，有91种（占7.5%）在两性或不同身体部位存在差异表达，这至少部分反映了特定的功能需求。目前已鉴定出43种蛋白质，约占检测到的蛋白质的3.5%。这些数据可通过可点击的内联凝胶图像和超文本链接从我们的万维网（WWW）服务器进行交互式访问。已鉴定的蛋白质斑点通过超文本链接与蛋白质一级序列的SWISS-PROT注释数据库以及果蝇基因组数据的Fly-Base数据库进行交叉引用。我们的参考凝胶可用于立即获取蛋白质斑点的鉴定信息以及黑腹果蝇中差异表达蛋白质的模式。本文介绍的工作将来自蛋白质二维聚丙烯酰胺凝胶电泳、分子生物学和遗传学的信息结合在一起，并提供了一种统一的方式来访问这些大量数据。
