Kitakawa M, Graack H R, Grohmann L, Goldschmidt-Reisin S, Herfurth E, Wittmann-Liebold B, Nishimura T, Isono K
Kobe University, Faculty of Science, Department of Biology, Rokkodai, Japan.
Eur J Biochem. 1997 Apr 15;245(2):449-56. doi: 10.1111/j.1432-1033.1997.t01-2-00449.x.
We have purified 13 large subunit proteins of the mitochondrial ribosome of the yeast Saccharomyces cerevisiae and determined their partial amino acid sequences. To elucidate the structure and function of these proteins, we searched for their genes by comparing our sequence data with those deduced from the genomic nucleotide sequence data of S. cerevisiae and analyzed them. In addition, we searched for the genes encoding proteins whose N-terminal amino acid sequences we have reported previously [Grohmann, L., Graack, H.-R., Kruft, V., Choli, T., Goldschmidt-Reisin, S. & Kitakawa, M. (1991) FEBS Lett. 284, 51-56]. Thus, we were able to identify and characterize 12 new genes for large subunit proteins of the yeast mitochondrial ribosome. Furthermore, we determined the N-terminal amino acid sequences of seven small subunit proteins and subsequently identified the genes for five of them, three of which were found to be new.
我们已经纯化了酿酒酵母线粒体核糖体的13种大亚基蛋白，并测定了它们的部分氨基酸序列。为了阐明这些蛋白质的结构和功能，我们通过将我们的序列数据与从酿酒酵母基因组核苷酸序列数据推导出来的序列进行比较来寻找它们的基因，并对其进行分析。此外，我们还搜索了编码我们之前报道过N端氨基酸序列的蛋白质的基因[格罗曼，L.，格拉阿克，H.-R.，克鲁夫特，V.，乔利，T.，戈德施密特-赖辛，S. & 北川，M.（1991）欧洲生物化学学会联合会快报。284，51 - 56]。因此，我们能够鉴定并表征酿酒酵母线粒体核糖体大亚基蛋白的12个新基因。此外，我们测定了7种小亚基蛋白的N端氨基酸序列，随后鉴定出其中5种的基因，其中3种被发现是新的。
