Hertveldt K, Philippé J, Thierens H, Cornelissen M, Vral A, De Ridder L
Department of Anatomy, Embryology and Histology, University of Ghent, Belgium.
Int J Radiat Biol. 1997 Apr;71(4):429-33. doi: 10.1080/095530097144049.
Human peripheral blood lymphocytes, irradiated in vitro, die by an apoptotic process. The number of apoptotic cells after in vitro gamma-irradiation (0, 0.1, 0.2, 1, 2 and 5 Gy) was measured by flow cytometry using Annexin V and DiOC6 (a cationic dye) after 24 and 48 h incubation. The mean dose-response curves for apoptosis of six healthy volunteers obtained with both methods were steep below 1 Gy and flatter at higher doses. A slightly higher number of apoptotic cells was observed with DiOC6, compared to Annexin V. This can be assigned to a minor DiOC6-int/PI- population. Forty-eight hour cultures contained higher numbers of apoptotic cells compared with 24 h cultures. For both culture times, DiOC6 and Annexin V detected a statistically significant difference between a control sample and a 0.1 Gy irradiated one, illustrating the high sensitivity of the methods.
体外照射的人外周血淋巴细胞通过凋亡过程死亡。在孵育24小时和48小时后，使用膜联蛋白V和DiOC6（一种阳离子染料）通过流式细胞术测量体外γ射线照射（0、0.1、0.2、1、2和5 Gy）后的凋亡细胞数量。用这两种方法获得的六名健康志愿者凋亡的平均剂量反应曲线在1 Gy以下较陡，在较高剂量时较平缓。与膜联蛋白V相比，用DiOC6观察到的凋亡细胞数量略多。这可归因于少量的DiOC6-int/PI-群体。与24小时培养物相比，48小时培养物中凋亡细胞数量更多。对于这两种培养时间，DiOC6和膜联蛋白V均检测到对照样品与0.1 Gy照射样品之间存在统计学上的显著差异，说明这些方法具有高灵敏度。
