Wang S Z, Smith P K, Lovejoy M, Bowden J J, Alpers J H, Forsyth K D
Department of Paediatrics, Flinders Medical Centre, Flinders University, Bedford Park, SA, Australia.
Clin Exp Immunol. 1998 Oct;114(1):49-54. doi: 10.1046/j.1365-2249.1998.00681.x.
Neutrophils are the predominant inflammatory cell in the lung tissues and airways in RSV infection, and can augment the epithelial cell damage induced by RSV. Neutrophil apoptosis has been suggested to be a mechanism to reduce the potential for tissue injury. The apoptosis of neutrophils from nasopharyngeal aspirates (NPA) (n = 19) and peripheral blood (PB) of infants with RSV bronchiolitis (n = 11) and PB from healthy controls (n = 9) was investigated. Monoclonal antibody against CD95 (Fas) and a binding protein Annexin V were used to determine the apoptosis of neutrophils. The expression of CD11b and CD18 on neutrophils was also detected with flow cytometry. The mean fluorescence intensity (MFI) of CD95 on neutrophils from RSV+ NPA was increased compared with cells from control PB (73.6 +/- 7.6 versus 31.5 +/- 4.3); the MFI of Annexin V, CD11b and CD18 on neutrophils from RSV+ NPA was up-regulated compared with cells from both control PB (105.3 +/- 18.1 versus 11.8 +/- 1.5; 1683 +/- 153.3 versus 841.1 +/- 72.3; 517 +/- 50.5 versus 147 +/- 8.7, respectively) and RSV+ PB (105.3 +/- 18.1 versus 35.8 +/- 4.1; 1683 +/- 153.3 versus 818 +/- 141.2; 517 +/- 50.5 versus 260 +/- 25.8, respectively). Furthermore, the percentage of neutrophils expressing Annexin V and the MFI of CD18 on neutrophils from RSV+ PB were increased compared with neutrophils from control PB. In addition, both CD11b (MFI) and CD18 (MFI) correlated with Annexin V (MFI) on neutrophils. We conclude that neutrophil apoptosis in RSV bronchiolitis is accelerated; and CD11b/CD18 may play an important role in RSV infection by influencing neutrophil apoptosis.
中性粒细胞是呼吸道合胞病毒（RSV）感染时肺组织和气道中主要的炎症细胞，可加重RSV诱导的上皮细胞损伤。中性粒细胞凋亡被认为是一种减少组织损伤可能性的机制。本研究调查了RSV细支气管炎婴儿（n = 11）的鼻咽抽吸物（NPA）（n = 19）和外周血（PB）以及健康对照者（n = 9）的PB中中性粒细胞的凋亡情况。使用抗CD95（Fas）单克隆抗体和结合蛋白膜联蛋白V来确定中性粒细胞的凋亡。还通过流式细胞术检测中性粒细胞上CD11b和CD18的表达。与对照PB中的细胞相比，RSV+ NPA中中性粒细胞上CD95的平均荧光强度（MFI）增加（73.6±7.6对31.5±4.3）；与对照PB和RSV+ PB中的细胞相比，RSV+ NPA中中性粒细胞上膜联蛋白V、CD11b和CD18的MFI均上调（分别为105.3±18.1对11.8±1.5；1683±153.3对841.1±72.3；517±50.5对147±8.7；以及105.3±18.1对35.8±4.1；1683±153.3对818±141.2；517±50.5对260±25.8）。此外，与对照PB中的中性粒细胞相比，RSV+ PB中表达膜联蛋白V的中性粒细胞百分比和中性粒细胞上CD18的MFI增加。另外，中性粒细胞上的CD11b（MFI）和CD18（MFI）均与膜联蛋白V（MFI）相关。我们得出结论，RSV细支气管炎中中性粒细胞凋亡加速；并且CD11b/CD18可能通过影响中性粒细胞凋亡在RSV感染中起重要作用。
