尼古丁可能会增强潜在牙周病原体毒素的致死性：对牙周疾病的影响
Possible lethal enhancement of toxins from putative periodontopathogens by nicotine: implications for periodontal disease.
作者信息
Sayers N M, Gomes B P, Drucker D B, Blinkhorn A S
机构信息
Turner Dental School, University of Manchester.
出版信息
J Clin Pathol. 1997 Mar;50(3):245-9. doi: 10.1136/jcp.50.3.245.
AIM
To test the hypothesis that lethal synergy in the chick embryo model may occur between nicotine and bacterial products (cell-free extracellular toxins and cell lysates) of five putative periodontopathogens.
METHODS
The lethality of cell-free extracellular toxins and cell lysates of five periodontal species was assessed with or without nicotine in the chick embryo assay system. Ten putative periodontopathogens (five species) were studied: Prevotella intermedia (n = 5), Porphyromonas gingivalis (n = 1), Porphyromonas asaccharolytica (n = 1), Fusobacterium nucleatum (n = 2), and Fusobacterium necrophorum (n = 1).
RESULTS
Simultaneous testing of cell-free extracellular toxins from isolates W50, PS2, PS3, PS4, and PS5 and nicotine resulted in a percentage kill significantly greater than expected (Fisher's Exact test). Simultaneous testing of cell lysates from isolates W50, PS2, and PS5 and nicotine resulted in a percentage kill significantly greater than expected (Fisher's Exact test).
CONCLUSIONS
Lethal synergy in the chick embryo model may occur between nicotine and toxins from putative periodontopathogens (both cell-free extracellular toxins and cell lysates). This may be an important mechanism by which smoking increases the severity of periodontal disease.
目的
验证尼古丁与五种假定牙周病原体的细菌产物（无细胞外毒素和细胞裂解物）在鸡胚模型中可能产生致死协同作用这一假说。
方法
在鸡胚试验系统中，评估有无尼古丁时五种牙周菌种的无细胞外毒素和细胞裂解物的致死性。研究了十种假定牙周病原体（五种菌种）：中间普氏菌（n = 5）、牙龈卟啉单胞菌（n = 1）、不解糖卟啉单胞菌（n = 1）、具核梭杆菌（n = 2）和坏死梭杆菌（n = 1）。
结果
对分离株W50、PS2、PS3、PS4和PS5的无细胞外毒素与尼古丁进行同步检测，导致的致死率显著高于预期（Fisher精确检验）。对分离株W50、PS2和PS5的细胞裂解物与尼古丁进行同步检测，导致的致死率显著高于预期（Fisher精确检验）。
结论
在鸡胚模型中，尼古丁与假定牙周病原体的毒素（无细胞外毒素和细胞裂解物）之间可能发生致死协同作用。这可能是吸烟增加牙周疾病严重程度的一个重要机制。
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