Shcherbata H R, Maksymiv D V
Tsitol Genet. 1997 Mar-Apr;31(2):44-9.
A novel system of prolonged genetic instability induced by N-ethyl-N-nitrosourea in D. melanogaster was obtained. The instability was associated with the appearance of new mutations in loci yellow, scute, white, cut, singed, miniature, vermilion and extended for 40 generations. Some mutants of locus cut were obtained by the method of Southern blot-hybridisation. The reversion ct+ appeared as a result of excision of gypsy element. The mutation ctpN34 was localized in the second complementation group of cut locus and was the consequent of deletions of gypsy element and small DNA fragment and an insertion of a DNA sequence 7.8 kb in size.
获得了一种由N-乙基-N-亚硝基脲在黑腹果蝇中诱导产生的新型长期遗传不稳定性系统。这种不稳定性与黄色、小盾片、白色、截翅、焦刚毛、微小翅、朱红色等基因座上新突变的出现有关，并持续了40代。通过Southern印迹杂交法获得了截翅基因座的一些突变体。回复突变ct+是由于gypsy元件的切除而出现的。突变ctpN34定位于截翅基因座的第二个互补群，是gypsy元件和小DNA片段缺失以及一个7.8 kb大小的DNA序列插入的结果。
