Shcherbata G R, Maksymiv D V
Genetika. 1997 Jan;33(1):19-24.
Molecular genetic mechanisms of the white mutations induced by mitomycin C, N-ethyl-N-nitrosourea, and ethidium bromide were studied. Genomic DNA of the original strain and mutants obtained was tested by Southern blot hybridization. The presence of mobile genetic elements was shown to be characteristic of the white locus of the original strain. Mutations of the white gene obtained mainly resulted from excision of DNA sequences involving mobile genetic elements and insertion of unidentified 5-6-kbp DNA fragments.
研究了丝裂霉素C、N-乙基-N-亚硝基脲和溴化乙锭诱导的白色突变的分子遗传机制。通过Southern印迹杂交检测原始菌株和获得的突变体的基因组DNA。结果表明，移动遗传元件的存在是原始菌株白色位点的特征。获得的白色基因突变主要是由于涉及移动遗传元件的DNA序列切除和未知的5-6kbp DNA片段插入所致。
