Ladvishchenko A B, Mogila V A, Georgiev P G, Simonova O B, Buff E M, Gerasimova T I
Genetika. 1990 Jul;26(7):1133-43.
The lines of the M'-cytotype characterized by a long-term instability (which was shown to be conditioned by transpositions of the new mobile element, Stalker) were hybridized with the P-line. This resulted in the appearance of a number of superunstable mutations at the yellow, white, singed, ocelliless and some other loci. The authors analyzed four independently obtained families of superunstable mutations at the singed locus. A wide spectrum of derivatives and high frequency of mutations were demonstrated, as well as the regularities of allelic transitions. Besides this, mutagenesis at the cut locus was observed in the chromosomes carrying sn mutations with frequency of 5.05 x 10(4). By means of the blot analysis it has been shown that most of ct mutations are intragenic deficiencies, ranging from 1.3 to 3 Kb, whose appearance is, conceivably, attributed to the inaccuracy of the insertion excision (the insertion is present but fails to alter the phenotype) at the cut locus of the chromosomes with the superunstable sn-alleles. In the lines with the sn- and ct-mutations the transpositions of the P-element and the Stalker were found, which indicates their involvement in mutagenesis. The authors discuss possible effects of inserting the complicated constructions, based on the combinations of P-element and the Stalker, on the induction of superinstability.
以长期不稳定性为特征的M'-细胞型品系（已证明这种不稳定性是由新的移动元件Stalker的转座作用所导致）与P-品系进行杂交。这导致在黄色、白色、焦刚毛、无眼及其他一些位点出现了许多超不稳定突变。作者分析了在焦刚毛位点独立获得的四个超不稳定突变家系。结果显示出广泛的衍生物谱和高突变频率，以及等位基因转变的规律。除此之外，在携带焦刚毛突变的染色体中观察到切口位点的诱变，频率为5.05×10⁻⁴。通过印迹分析表明，大多数切口突变是基因内缺失，范围从1.3到3千碱基对，其出现可能归因于具有超不稳定焦刚毛等位基因的染色体在切口位点的插入-切除不准确（插入存在但未改变表型）。在具有焦刚毛和切口突变的品系中发现了P-元件和Stalker的转座，这表明它们参与了诱变作用。作者讨论了基于P-元件和Stalker的组合插入复杂结构对诱导超不稳定性可能产生的影响。
