Clark S E, Williams R W, Meyerowitz E M
Division of Biology, California Institute of Technology, Pasadena 91125, USA.
Cell. 1997 May 16;89(4):575-85. doi: 10.1016/s0092-8674(00)80239-1.
The shoot apical meristem is responsible for above-ground organ initiation in higher plants, accomplishing continuous organogenesis by maintaining a pool of undifferentiated cells and directing descendant cells toward organ formation. Normally, proliferation and differentiation are balanced, so that the structure and size of the shoot meristem is maintained. However, Arabidopsis plants homozygous for mutations at the CLAVATA1 (CLV1) locus accumulate excess undifferentiated cells. We describe the molecular cloning and expression pattern of the CLV1 gene. It encodes a putative receptor kinase, suggesting a role in signal transduction. The extracellular domain is composed of 21 tandem leucine-rich repeats that resemble leucine-rich repeats found in animal hormone receptors. We provide evidence that CLV1 expression in the inflorescence is specifically associated with meristematic activity.
茎尖分生组织负责高等植物地上器官的起始，通过维持一群未分化细胞并引导后代细胞向器官形成方向发展来完成连续的器官发生。正常情况下，增殖和分化是平衡的，从而维持茎尖分生组织的结构和大小。然而，CLAVATA1（CLV1）基因座发生突变的纯合拟南芥植株会积累过量的未分化细胞。我们描述了CLV1基因的分子克隆及表达模式。它编码一种假定的受体激酶，表明其在信号转导中起作用。细胞外结构域由21个串联的富含亮氨酸重复序列组成，类似于动物激素受体中发现的富含亮氨酸重复序列。我们提供的证据表明，CLV1在花序中的表达与分生组织活性特异性相关。
