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[移动元件hobo在黑腹果蝇长期不稳定系统转座事件中的参与]

[Participation of mobile element hobo in transposition events in the long-term instability system of Drosophila melanogaster].

作者信息

Buff E M, Simonova O B, Petruk S F, Gerasimova T I

出版信息

Genetika. 1992 Dec;28(12):73-9.

PMID:1338197
Abstract

The lines with an active hobo elements as well as those without any hobo fragments were hybridized with the y2sc1waG line. This resulted in the appearance of a number of mutations at the white, miniature, and some other loci. The authors analysed, in which way the hobo transposable elements take part in mutagenesis in these crosses. Most of the white mutants obtained were analysed and transpositions of hobo and Stalker elements were demonstrated. Both independent and simultaneous transpositions were found. It was shown by means of the Southern blot analysis that additional hobo or Stalker insertion into or close to the parental unknown waG insertion resulted in mutant white phenotype's shift toward both extreme and partial reversion. Possible participation in mutagenesis of other mobile elements is also under debate.

摘要

具有活跃hobo元件的品系以及没有任何hobo片段的品系与y2sc1waG品系进行杂交。这导致在白眼、小型化及其他一些位点出现了许多突变。作者分析了hobo转座元件在这些杂交中参与诱变的方式。对获得的大多数白眼突变体进行了分析,并证实了hobo和Stalker元件的转座。发现了独立转座和同时转座。通过Southern印迹分析表明,在亲本未知的waG插入位点或其附近额外插入hobo或Stalker会导致突变的白眼表型向极端和部分回复转变。其他移动元件在诱变中的可能参与也在讨论中。

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