Philippsen P, Kleine K, Pöhlmann R, Düsterhöft A, Hamberg K, Hegemann J H, Obermaier B, Urrestarazu L A, Aert R, Albermann K, Altmann R, André B, Baladron V, Ballesta J P, Bécam A M, Beinhauer J, Boskovic J, Buitrago M J, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo J M, Glansdorff N N, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux J L, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer K C, Messenguy F, Mewes H W, Mösti D, Nasr F, Nicaud J M, Niedenthal R K, Pandolfo D, Piérard A, Piravandi E, Planta R J, Pohl T M, Purnelle B, Rebischung C, Remacha M, Revuelta J L, Rinke M, Saiz J E, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus J H, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Institute for Applied Microbiology, Biozentrum, University of Basel, Switzerland.
Nature. 1997 May 29;387(6632 Suppl):93-8.
In 1992 we started assembling an ordered library of cosmid clones from chromosome XIV of the yeast Saccharomyces cerevisiae. At that time, only 49 genes were known to be located on this chromosome and we estimated that 80% to 90% of its genes were yet to be discovered. In 1993, a team of 20 European laboratories began the systematic sequence analysis of chromosome XIV. The completed and intensively checked final sequence of 784,328 base pairs was released in April, 1996. Substantial parts had been published before or had previously been made available on request. The sequence contained 419 known or presumptive protein-coding genes, including two pseudogenes and three retrotransposons, 14 tRNA genes, and three small nuclear RNA genes. For 116 (30%) protein-coding sequences, one or more structural homologues were identified elsewhere in the yeast genome. Half of them belong to duplicated groups of 6-14 loosely linked genes, in most cases with conserved gene order and orientation (relaxed interchromosomal synteny). We have considered the possible evolutionary origins of this unexpected feature of yeast genome organization.
1992年，我们开始构建来自酿酒酵母第十四号染色体的黏粒克隆有序文库。当时，已知仅49个基因位于这条染色体上，我们估计其80%至90%的基因尚未被发现。1993年，一个由20个欧洲实验室组成的团队开始对第十四号染色体进行系统的序列分析。1996年4月发布了完成并经过严格检查的784,328个碱基对的最终序列。其中大部分内容之前已发表或之前可应要求提供。该序列包含419个已知或推测的蛋白质编码基因，包括两个假基因和三个逆转座子、14个tRNA基因以及三个小核RNA基因。对于116个（30%）蛋白质编码序列，在酵母基因组的其他地方鉴定出了一个或多个结构同源物。其中一半属于6至14个松散连锁基因的重复组，在大多数情况下具有保守的基因顺序和方向（松弛的染色体间同线性）。我们已经考虑了酵母基因组组织这一意外特征可能的进化起源。
