Bussey H, Storms R K, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Brückner M, Carpenter J, Cherry J M, Chung E, Churcher C, Coster F, Davis K, Davis R W, Dietrich F S, Delius H, DiPaolo T, Hani J
Department of Biology, McGill University, Montreal, Canada.
Nature. 1997 May 29;387(6632 Suppl):103-5.
The nucleotide sequence of the 948,061 base pairs of chromosome XVI has been determined, completing the sequence of the yeast genome. Chromosome XVI was the last yeast chromosome identified, and some of the genes mapped early to it, such as GAL4, PEP4 and RAD1 (ref. 2) have played important roles in the development of yeast biology. The architecture of this final chromosome seems to be typical of the large yeast chromosomes, and shows large duplications with other yeast chromosomes. Chromosome XVI contains 487 potential protein-encoding genes, 17 tRNA genes and two small nuclear RNA genes; 27% of the genes have significant similarities to human gene products, and 48% are new and of unknown biological function. Systematic efforts to explore gene function have begun.
16号染色体948,061个碱基对的核苷酸序列已被确定，从而完成了酵母基因组的测序。16号染色体是最后一个被鉴定的酵母染色体，一些早期定位到该染色体上的基因，如GAL4、PEP4和RAD1（参考文献2）在酵母生物学的发展中发挥了重要作用。这条最终染色体的结构似乎是大型酵母染色体的典型结构，并且与其他酵母染色体存在大量重复。16号染色体包含487个潜在的蛋白质编码基因、17个tRNA基因和两个小核RNA基因；27%的基因与人类基因产物有显著相似性，48%是新基因且其生物学功能未知。探索基因功能的系统性工作已经开始。
