Myers S R, Grady J, Soranzo C, Sanders R, Green C, Leigh I M, Navsaria H A
Restoration of Appearance and Function Trust (RAFT), Mount Vernon Hospital, Middlesex, United Kingdom.
J Burn Care Rehabil. 1997 May-Jun;18(3):214-22. doi: 10.1097/00004630-199705000-00007.
The clinical take rates of cultured keratinocyte autografts are poor on a full-thickness wound unless a dermal bed is provided. Even under these circumstances two important problems are the time delay in growing autografts and the fragility of the grafts. A laser-perforated hyaluronic acid membrane delivery system allows grafting at early confluence without requiring dispase digestion to release grafts from their culture dishes. We designed this study to investigate the influence of this membrane on clinical take rates in an established porcine kerato-dermal grafting model. The study demonstrated a significant reduction in take as a result of halving the keratinocyte seeding density onto the membrane. The take rates, however, of grafts grown on the membrane at half or full conventional seeding density and transplanted to a dermal wound bed were comparable, if not better, than those of keratinocyte sheet grafts.
除非提供真皮床，培养的自体角质形成细胞移植片在全层伤口上的临床成活率很低。即使在这些情况下，仍存在两个重要问题，即自体移植片生长的时间延迟和移植片的脆弱性。一种激光打孔透明质酸膜递送系统允许在早期汇合时进行移植，而无需用分散酶消化以将移植片从培养皿中释放出来。我们设计了这项研究，以在既定的猪角膜-皮肤移植模型中研究这种膜对临床成活率的影响。该研究表明，由于将角质形成细胞接种到膜上的密度减半，成活率显著降低。然而，以常规接种密度的一半或全部接种在膜上并移植到真皮伤口床的移植片的成活率，即使不比角质形成细胞片移植的成活率更好，也与之相当。
