Oketch-Rabah H A, Lemmich E, Dossaji S F, Theander T G, Olsen C E, Cornett C, Kharazmi A, Christensen S B
Phytochemistry Department, National Museums of Kenya, Nairobi, Kenya.
J Nat Prod. 1997 May;60(5):458-61. doi: 10.1021/np970030o.
Two new isomeric 5-methylcoumarins, 2'-epicycloisobrachycoumarinone epoxide (1) and cycloisobrachycoumarinone epoxide (2), have been isolated from the roots of Vernonia brachycalyx by means of bioactivity-guided fractionation. The structures were elucidated by MS and NMR spectroscopic methods. Compounds 1 and 2 showed in vitro activity against Leishmania major promastigotes and against Plasmodium falciparum schizonts and demonstrated an inhibition on the proliferation of human lymphocytes, which was significantly weaker than the antiparasitic effects.
通过生物活性导向分离法，从短萼斑鸠菊的根中分离出了两种新的异构5-甲基香豆素，即2'-表环异短叶斑鸠菊酮环氧化物（1）和环异短叶斑鸠菊酮环氧化物（2）。通过质谱和核磁共振光谱方法对其结构进行了阐明。化合物1和2对硕大利什曼原虫前鞭毛体和恶性疟原虫裂殖体显示出体外活性，并对人淋巴细胞的增殖有抑制作用，但其抑制作用明显弱于抗寄生虫作用。
