Wang S, Zang L, Feng M, Liang Z, Wang S, Zheng S, Zhang L, Jiang Z, Chen D
Zhengzhou 460 Hospital, China.
Ultrastruct Pathol. 1997 May-Jun;21(3):281-7. doi: 10.3109/01913129709021924.
Epidemic hemorrhagic fever (EHF) virus particles were found in the squamous epithelial cells and the capillary endothelial cells of the petechial spots located on the mucous membrane of the soft palate in 3 patients with severe early-stage EHF with transmission electron microscopy. The virus particles were round or oval in shape, about 100 nm in diameter, with a two-layer lipid envelope from which spikes were protruding. The nucleocapsid of the virus appeared to be hollow microfilamentoid or dense granules. Meanwhile, budding virus particles with a diameter of 80 nm were found in the enlarged Golgi apparatus. The infected cells displayed an enlarged and proliferating Golgi apparatus. The morphological characteristics of the viron mentioned above coincided with those of the virus particles of the family Bunyaviridae. This study is the first to demonstrate that the squamous epithelial cells on the mucous membrane of the soft palate are the target cells of EHF infection and to provide subcellular morphological evidence of petechial hemorrhage at the soft palate.
应用透射电子显微镜，在3例早期重症流行性出血热患者软腭黏膜瘀点处的鳞状上皮细胞及毛细血管内皮细胞中发现了流行性出血热（EHF）病毒颗粒。病毒颗粒呈圆形或椭圆形，直径约100nm，有双层脂质包膜，包膜上有刺突伸出。病毒核衣壳呈中空微丝状或致密颗粒状。同时，在肿大的高尔基体中发现了直径为80nm的出芽病毒颗粒。受感染细胞的高尔基体肿大并增殖。上述病毒体的形态特征与布尼亚病毒科病毒颗粒的形态特征相符。本研究首次证明软腭黏膜的鳞状上皮细胞是EHF感染的靶细胞，并为软腭瘀点出血提供了亚细胞形态学证据。
