Tarazona R, Lally N C, Dominguez-Carmona M, Blewett D A
Departamento de medicina Preventiva, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Int J Parasitol. 1997 Apr;27(4):417-23. doi: 10.1016/s0020-7519(96)00189-0.
Mice inoculated at 5, 21 and 28 days of age with 10(6) or 10(7) Cryptosporidium parvum oocysts became infected but did not exhibit any clinical signs of disease. Specific IgA antibodies were detected in faecal extracts from all infected mice by an indirect immunofluorescent assay. These antibodies first appeared between 11 and 37 days post-infection (dpi) and persisted until the end of the experiment at 55 dpl. They appeared earlier in older mice than in newborn mice. Reduction and resolution of oocyst shedding was not directly related, however, to IgA antibody levels in infected mice. Reactive C. parvum antigens were identified by immunoblotting techniques using faecal and serum samples from infected mice. IgA copro-antibodies reacted specifically with two antigens of 26 and 33 kDa, which were also identified by IgG antibodies in mouse serum. The role of these antibodies in the resolution of infections and the subsequent protection against challenge is unknown.
在5日龄、21日龄和28日龄时接种10⁶或10⁷个微小隐孢子虫卵囊的小鼠受到了感染,但未表现出任何疾病的临床症状。通过间接免疫荧光测定法在所有受感染小鼠的粪便提取物中检测到了特异性IgA抗体。这些抗体在感染后11至37天首次出现,并持续到实验结束时的第55天。它们在年龄较大的小鼠中比在新生小鼠中出现得更早。然而,卵囊排出的减少和消失与受感染小鼠中的IgA抗体水平没有直接关系。使用受感染小鼠的粪便和血清样本,通过免疫印迹技术鉴定出了反应性微小隐孢子虫抗原。IgA粪便抗体与26 kDa和33 kDa的两种抗原发生特异性反应,这两种抗原也在小鼠血清中的IgG抗体中得到鉴定。这些抗体在感染消退以及随后抵御攻击方面所起的作用尚不清楚。