Reperant J M, Naciri M, Chardes T, Bout D T
Institut National de la Recherche Agronomique (I.N.R.A.), Nouzilly, France.
FEMS Microbiol Lett. 1992 Nov 15;78(1):7-14. doi: 10.1016/0378-1097(92)90280-2.
Cryptosporidium parvum antigens were characterized by immunoblot analysis of sera and intestinal secretions of BALB/c mice orally infected with 10(5) oocysts. A major band at 17 kDa under non-reduced conditions and at 18 kDa under reduced conditions was recognized by anti-C. parvum IgA and IgG in serum and intestinal secretions from day 15 post-infection. This recognition persisted throughout the experiment (day 30). Mouse-serum antibodies raised against the 17-kDa purified antigen (P17) showed no cross-reactivity with other C. parvum antigens. Immunofluorescence study revealed that this antigen is located on the sporozoite. It is suggested that this antigen could be a good candidate for studies of mucosal immune response to C. parvum and for vaccination.
通过对口服感染10⁵个卵囊的BALB/c小鼠的血清和肠道分泌物进行免疫印迹分析，对微小隐孢子虫抗原进行了表征。在感染后第15天，血清和肠道分泌物中的抗微小隐孢子虫IgA和IgG识别出非还原条件下17 kDa和还原条件下18 kDa的一条主要条带。这种识别在整个实验过程中（第30天）持续存在。针对17 kDa纯化抗原（P17）产生的小鼠血清抗体与其他微小隐孢子虫抗原无交叉反应。免疫荧光研究表明，该抗原位于子孢子上。提示该抗原可能是研究对微小隐孢子虫黏膜免疫反应及疫苗接种的良好候选抗原。
