Ehrsam A, Coval G, Betuel H
Rev Fr Transfus Immunohematol. 1977 Sep;20(3):483-5. doi: 10.1016/s0338-4535(77)80082-2.
After centrifugation of 450 ml-CPD-whole blood, 125 ml of packed red cells are diluted to 50 percent with saline (pH 7, 2). To remove leukocytes the diluted blood is filtered at room temperature through a newly devised column packed with cotton wool disks. 200 ml of red blood cells suspension are recovered and contain less than 5.10(6) leukocytes. Appropriate controls show that: 1. The filtered blood contains no pyrogen; 2. The red blood cells have a normal osmotic resistance; 3. The suspension can be maintained at 4 degrees C up to 5 days before transfusion without cellular damage.
将450毫升CPD全血离心后，125毫升压紧红细胞用生理盐水（pH值7.2）稀释至50%。为去除白细胞，将稀释后的血液在室温下通过一个新设计的装有脱脂棉盘的柱进行过滤。回收得到200毫升红细胞悬液，其中白细胞含量少于5×10⁶个。适当的对照显示：1. 过滤后的血液无热原；2. 红细胞具有正常的渗透抵抗力；3. 该悬液在4℃下可保存至输血前5天而无细胞损伤。
