Romero C H, Barrett T, Chamberlain R W, Kitching R P, Fleming M, Black D N
Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, United Kingdom.
Virology. 1994 Oct;204(1):425-9. doi: 10.1006/viro.1994.1548.
A cDNA clone containing the complete coding sequence of the hemagglutinin (H) protein gene of the RBOK vaccine strain of rinderpest virus, under the control of the vaccinia late promoter p11, was inserted by homologous recombination into the thymidine kinase gene of the KS-1 strain of capripoxvirus. The recombinant virus produced authentic H protein as judged by its electrophoretic mobility, transport to the cell surface of infected lamb testis cells, and reactivity with monoclonal antibodies specific for the H protein of rinderpest virus. The recombinant virus induced significant levels of rinderpest virus neutralizing antibodies in vaccinated cattle and protected them from clinical rinderpest after challenge with a lethal dose of a highly virulent heterologous strain of the virus. Protection was achieved using vaccine doses lower than those used with a similar recombinant expressing the fusion protein gene of rinderpest. The parental KS-1 virus is widely used as a vaccine against capripox viruses and so the rinderpest recombinant acts as a dual vaccine to protect cattle against both rinderpest and lumpy skin disease.
在痘苗晚期启动子p11的控制下，将含有牛瘟病毒RBOK疫苗株血凝素（H）蛋白基因完整编码序列的cDNA克隆，通过同源重组插入到山羊痘病毒KS-1株的胸苷激酶基因中。通过重组病毒的电泳迁移率、其向感染的羔羊睾丸细胞表面的转运以及与牛瘟病毒H蛋白特异性单克隆抗体的反应性判断，该重组病毒产生了天然的H蛋白。该重组病毒在接种疫苗的牛中诱导产生了高水平的牛瘟病毒中和抗体，并在用致死剂量的高毒力异源病毒株攻击后保护它们免受临床牛瘟的侵害。使用低于表达牛瘟融合蛋白基因的类似重组体所使用的疫苗剂量即可实现保护。亲本KS-1病毒被广泛用作抗山羊痘病毒的疫苗，因此牛瘟重组体可作为一种双价疫苗，保护牛免受牛瘟和结节性皮肤病的侵害。
