Leong A S
Division of Tissue Pathology, Institute of Medical and Veterinary Science, Adelaide, South Australia.
Pathology. 1991 Jul;23(3):271-3. doi: 10.3109/00313029109063583.
Technical details of a method of microwave fixation and rapid processing are described. Fresh biopsy specimens are sampled and trimmed into 2 mm thick blocks and irradiated to a temperature of 68 degrees C. They are then processed through cycles of absolute alcohol (75 min), xylene (50 min) and wax (50 min). It is possible, in this manner, to run several 'short' cycles of about 3 hrs each during the working day so that stained sections are available on the same day as receipt of the specimens. Endoscopic biopsies are processed through a shorter cycle comprising 30 min of absolute alcohol (4 changes), 20 min of xylene (3 changes) and 20 min of wax (2 changes). For convenience we also employ an overnight cycle whereby microwave-fixed blocks are processed through several changes of absolute alcohol, xylene and wax without the use of formalin. This method of processing not only removes the use of noxious and potentially toxic formalin but also allows rapid preparation of good quality diagnostic sections with superior antigen preservation compared to formaldehyde fixation.
本文描述了一种微波固定和快速处理方法的技术细节。取新鲜活检标本，切成2毫米厚的组织块，照射至68摄氏度。然后依次经过无水乙醇（75分钟）、二甲苯（50分钟）和石蜡（50分钟）处理。采用这种方法，工作日期间可以运行几个约3小时的“短”周期，以便在收到标本的同一天获得染色切片。内镜活检组织经过较短的周期处理，包括无水乙醇30分钟（更换4次）、二甲苯20分钟（更换3次）和石蜡20分钟（更换2次）。为方便起见，我们还采用过夜处理周期，即微波固定的组织块不使用福尔马林，经过多次无水乙醇、二甲苯和石蜡更换处理。这种处理方法不仅避免了使用有毒且有潜在毒性的福尔马林，而且与甲醛固定相比，能够快速制备高质量的诊断切片，同时具有更好的抗原保存效果。
