玉米VP14对类胡萝卜素的特异性氧化裂解

Specific oxidative cleavage of carotenoids by VP14 of maize.

作者信息

Schwartz S H, Tan B C, Gage D A, Zeevaart J A, McCarty D R

机构信息

Michigan State University-Department of Energy (MSU-DOE) Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA.

出版信息

Science. 1997 Jun 20;276(5320):1872-4. doi: 10.1126/science.276.5320.1872.

DOI:10.1126/science.276.5320.1872

PMID:9188535

Abstract

The plant growth regulator abscisic acid (ABA) is formed by the oxidative cleavage of an epoxy-carotenoid. The synthesis of other apocarotenoids, such as vitamin A in animals, may occur by a similar mechanism. In ABA biosynthesis, oxidative cleavage is the first committed reaction and is believed to be the key regulatory step. A new ABA-deficient mutant of maize has been identified and the corresponding gene, Vp14, has been cloned. The recombinant VP14 protein catalyzes the cleavage of 9-cis-epoxy-carotenoids to form C25 apo-aldehydes and xanthoxin, a precursor of ABA in higher plants.

摘要

植物生长调节剂脱落酸（ABA）由环氧类胡萝卜素的氧化裂解形成。其他类胡萝卜素的合成，如动物体内的维生素A，可能通过类似的机制发生。在ABA生物合成中，氧化裂解是第一个关键反应，被认为是关键的调控步骤。已鉴定出一种新的玉米ABA缺陷型突变体，并克隆了相应的基因Vp14。重组VP14蛋白催化9-顺式环氧类胡萝卜素的裂解，形成C25脱辅醛和黄质醛，黄质醛是高等植物中ABA的前体。