Yanagawa Y, Takahashi M, Itoh T
Tokyo Metropolitan Research Laboratory of Public Health.
Nihon Saikingaku Zasshi. 1994 Mar;49(2):395-403. doi: 10.3412/jsb.49.395.
For analyzing the role of the bacterial flagella in colonization in the intestinal tract of mice and adhering to or invading the Intestine 407 cell, a nonflagellated, nonmotile mutant was induced by ultraviolet irradiation of a flagellated, motile wild-type strain of Campylobacter jejuni CF84-340. There was no great difference in the cellular infectivity to the Intestine 407 cells between the wild-type and the mutant strains. Cellular adherence and invasiveness were then compared by fluorescent antibody staining, and an obvious difference was found in the latter. While 21.4% of the organisms of the wied-type strain invaded the cells, only 6.1% of those of the flagella-defective mutant did so. In the experiments in mice involving oral administration, cellular invasiveness was not found with the flagella-defective mutant and no organisms were detected from the blood, although bacteremia is one of the characteristics of infection with C. jejuni. Moreover, no intestinal adherence of the mutant was detected, suggesting early elimination of the organism administered. These results indicate that the bacterial flagella are concerned in not only the cellular adherence and intestinal deposit, but also the intracellular invasiveness and invasion into the blood stream from the intestinal wall in the infected mice.
为了分析细菌鞭毛在小鼠肠道定植以及黏附或侵袭肠407细胞中的作用，通过对空肠弯曲菌CF84 - 340的鞭毛运动野生型菌株进行紫外线照射，诱导出了无鞭毛、无运动能力的突变体。野生型菌株和突变体菌株对肠407细胞的细胞感染性没有很大差异。然后通过荧光抗体染色比较细胞黏附性和侵袭性，发现后者存在明显差异。野生型菌株中有21.4%的菌体侵袭细胞，而鞭毛缺陷突变体中只有6.1%的菌体如此。在涉及口服给药的小鼠实验中，鞭毛缺陷突变体未发现细胞侵袭性，且血液中未检测到菌体，尽管菌血症是空肠弯曲菌感染的特征之一。此外，未检测到突变体在肠道的黏附，这表明所给予的菌体被早期清除。这些结果表明，细菌鞭毛不仅与细胞黏附和肠道定植有关，还与感染小鼠中细胞内侵袭以及从肠壁侵入血流有关。
