Henriques Marília, Quintas Célia, Loureiro-Dias Maria C
Laboratory of Microbiology, Gulbenkian Institute of Science, Ap. 14, 2781 Oeiras Codex, Portugal.
Microbiology (Reading). 1997 Jun;143 ( Pt 6):1877-1883. doi: 10.1099/00221287-143-6-1877.
When grown in the presence of benzoic acid, Saccharomyces cerevisiae was able to extrude [(14)C]benzoic acid when a pulse a glucose was given to preloaded cells. While octanoic, sorbic, hexanoic, salicylic, butyric and propionic acids were also inducers, ethanol and acetic acid were not. The mechanism of extrusion required energy and prior growth in the presence of the inducers. Diethylstilbestrol, an inhibitor of ATPases, prevented benzoic acid extrusion. Propionic acid was not actively extruded in cells adapted to either benzoic or propionic acid, behaving as an appropriate probe to measure intracellular pH. Even though the extrusion mechanism was active, benzoic acid entered the cells by a simple diffusion mechanism.
当在苯甲酸存在的情况下培养时，给预先加载了葡萄糖的酿酒酵母细胞施加一次葡萄糖脉冲，它能够排出[¹⁴C]苯甲酸。虽然辛酸、山梨酸、己酸、水杨酸、丁酸和丙酸也是诱导剂，但乙醇和乙酸不是。排出机制需要能量以及在诱导剂存在的情况下预先生长。ATP酶抑制剂己烯雌酚可阻止苯甲酸的排出。丙酸在适应苯甲酸或丙酸的细胞中不会被主动排出，可作为测量细胞内pH的合适探针。尽管排出机制是活跃的，但苯甲酸通过简单扩散机制进入细胞。
