Ellmerer M, Schaupp L, Sendlhofer G, Wutte A, Brunner G A, Trajanoski Z, Skrabal F, Wach P, Pieber T R
Department of Internal Medicine and Diabetes and Metabolism, Karl Franzens University Graz, Austria.
J Clin Endocrinol Metab. 1998 Dec;83(12):4394-401. doi: 10.1210/jcem.83.12.5303.
Open flow microperfusion and a novel calibration technique (ionic reference technique) were evaluated for the frequent measurement of the absolute lactate concentration in sc adipose tissue. Furthermore, the influence of the plasma insulin concentration on the lactate concentration of sc adipose tissue was investigated during hyperglycemia. Sixteen lean healthy young men participated in the studies. In the postabsorbtive state the mean sc lactate concentrations were 1.29 and 1.36 mmol/L for the ionic reference technique and the no net flux protocol, respectively (not significant, P > 0.05). The simultaneously measured arterialized plasma lactate concentration was significantly lower at 0.77 mmol/L (P < 0.05). Both the sc lactate concentration (1.8+/-0.33 mmol/L) and the plasma lactate concentration (0.96+/-0.03 mmol/L) were significantly elevated during a hyperinsulinemic euglycemic clamp experiment. During a hyperglycemic clamp experiment the sc lactate concentration reached a significantly elevated plateau (2.15+/-0.27 mmol/L) that was not influenced by the increasing plasma insulin concentration. It is concluded that 1) open flow microperfusion combined with the ionic reference technique enables frequent measurement of the sc lactate concentration; 2) sc adipose tissue is a significant source of lactate release in the postabsorbtive state as well as during hyperinsulinemic clamp conditions; and 3) insulin concentrations greater than 180 pmol/L have no further influence on adipocyte stimulation of sc adipose tissue with respect to lactate release.
对开放流动微灌注和一种新型校准技术（离子参考技术）进行了评估，以用于频繁测量皮下脂肪组织中的绝对乳酸浓度。此外，还研究了高血糖期间血浆胰岛素浓度对皮下脂肪组织乳酸浓度的影响。16名健康瘦的年轻男性参与了这些研究。在吸收后状态下，离子参考技术和无净通量方案的皮下乳酸平均浓度分别为1.29和1.36 mmol/L（无显著差异，P>0.05）。同时测量的动脉化血浆乳酸浓度显著较低，为0.77 mmol/L（P<0.05）。在高胰岛素正常血糖钳夹实验期间，皮下乳酸浓度（1.8±0.33 mmol/L）和血浆乳酸浓度（0.96±0.03 mmol/L）均显著升高。在高血糖钳夹实验期间，皮下乳酸浓度达到显著升高的平台期（2.15±0.27 mmol/L），且不受血浆胰岛素浓度升高的影响。得出以下结论：1）开放流动微灌注结合离子参考技术能够频繁测量皮下乳酸浓度；2）皮下脂肪组织在吸收后状态以及高胰岛素钳夹条件下是乳酸释放的重要来源；3）胰岛素浓度大于180 pmol/L对皮下脂肪组织中脂肪细胞乳酸释放的刺激没有进一步影响。
