Kinoshita T, Miyake K, Nakao H, Tanaka T, Tsuda F, Okamoto H, Miyakawa Y, Mayumi M
First Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan.
J Infect Dis. 1997 Feb;175(2):454-7. doi: 10.1093/infdis/175.2.454.
RNA of a putative non-A, -B, -C, -D, or -E hepatitis virus named GB virus C (GBV-C) was detected by reverse transcription-polymerase chain reaction with primers deduced from the 5' untranslated region in 15 (24%) of 63 men with hemophilia in Japan at a frequency higher (P < .001) than that in 2 (0.6%) of 337 controls. By phylogenetic analysis, GBV-C isolates from some patients were similar in sequence, indicating infection with closely related strains, and those from certain patients resembled sequences reported from foreign countries. All patients were infected with hepatitis C virus, and genotypes that are rare in Japan were detected in 36 (57%) of them. These results indicate that patients with hemophilia in Japan would be at increased risk for infection with GBV-C and hepatitis C virus, some of which would have been transmitted via imported coagulation factor concentrates in the past.
采用逆转录聚合酶链反应，以从5'非翻译区推导的引物，在日本63名血友病男性患者中的15名（24%）检测到一种名为GB病毒C（GBV-C）的假定非甲、非乙、非丙、非丁、非戊型肝炎病毒的RNA，其频率高于（P < 0.001）337名对照中的2名（0.6%）。通过系统发育分析，一些患者的GBV-C分离株序列相似，表明感染了密切相关的毒株，而某些患者的分离株序列与国外报道的序列相似。所有患者均感染丙型肝炎病毒，其中36名（57%）检测到在日本罕见的基因型。这些结果表明，日本的血友病患者感染GBV-C和丙型肝炎病毒的风险增加，其中一些可能是过去通过进口凝血因子浓缩物传播的。
