Dille B J, Surowy T K, Gutierrez R A, Coleman P F, Knigge M F, Carrick R J, Aach R D, Hollinger F B, Stevens C E, Barbosa L H, Nemo G J, Mosley J W, Dawson G J, Mushahwar I K
Viral Discovery Group, Abbott Laboratories, North Chicago, Illinois 60064, USA.
J Infect Dis. 1997 Feb;175(2):458-61. doi: 10.1093/infdis/175.2.458.
An ELISA was developed for detection of antibodies to GB virus C (GBV-C) using a recombinant E2 protein expressed in CHO cells. Seroconversion to anti-E2 positivity was noted among several persons infected with GBV-C RNA-positive blood through transfusion. Of 6 blood recipients infected by GBV-C RNA-positive donors, 4 (67%) became anti-E2 positive and cleared their viremia. Thus, anti-E2 seroconversion is associated with viral clearance. The prevalence of antibodies to E2 was relatively low (3.0%-8.1%) in volunteer blood donors but was higher in several other groups, including plasmapheresis donors (34.0%), intravenous drug users (85.2%), and West African subjects (13.3%), all of whom tested negative by GBV-C reverse-transcription polymerase chain reaction (RT-PCR). These data demonstrate that testing for anti-E2 should greatly extend the ability of RT-PCR to define the epidemiology and clinical significance of GBV-C.
利用在CHO细胞中表达的重组E2蛋白开发了一种用于检测丙型肝炎病毒（GBV-C）抗体的酶联免疫吸附测定（ELISA）。在几名因输血感染GBV-C RNA阳性血液的人中发现了抗E2阳性的血清转化。在6名受GBV-C RNA阳性供体感染的输血接受者中，4名（67%）抗E2呈阳性并清除了病毒血症。因此，抗E2血清转化与病毒清除有关。抗E2抗体在志愿献血者中的流行率相对较低（3.0%-8.1%），但在其他几个群体中较高，包括单采血浆供血者（34.0%）、静脉吸毒者（85.2%）和西非受试者（13.3%），所有这些人通过GBV-C逆转录聚合酶链反应（RT-PCR）检测均为阴性。这些数据表明，抗E2检测应能大大扩展RT-PCR在确定GBV-C流行病学和临床意义方面的能力。
