缺乏POU蛋白精子-1的小鼠生育力降低。
Reduced fertility in mice deficient for the POU protein sperm-1.
作者信息
Pearse R V, Drolet D W, Kalla K A, Hooshmand F, Bermingham J R, Rosenfeld M G
机构信息
Eukaryotic Regulatory Biology Program, Howard Hughes Medical Institute, Cellular and Molecular Medicine, University of California, San Diego, CA 92093-0648, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7555-60. doi: 10.1073/pnas.94.14.7555.
Abstract
Members of the POU-homeodomain gene family encode transcriptional regulatory molecules that play important roles in terminal differentiation of many organ systems. Sperm-1 (Sprm-1) is a POU domain factor that is exclusively expressed in the differentiating male germ cell. We show here that the Sprm-1 protein is expressed in the haploid spermatid and that 129/Sv Sprm-1(-/-) mice are subfertile when compared with wild-type or heterozygous littermates yet exhibit normal testicular morphology and produce normal numbers of mobile spermatozoa. Our data suggest that the Sprm-1 protein plays a discrete regulatory function in the haploid spermatid, which is required for the optimal function, but not the terminal differentiation, of the male germ cell.
摘要
POU 同源结构域基因家族的成员编码转录调节分子，这些分子在许多器官系统的终末分化中发挥重要作用。精子 1（Sprm - 1）是一种 POU 结构域因子，仅在分化中的雄性生殖细胞中表达。我们在此表明，Sprm - 1 蛋白在单倍体精子细胞中表达，并且与野生型或杂合子同窝小鼠相比，129/Sv Sprm - 1(-/-)小鼠生育力低下，但睾丸形态正常且产生正常数量的活动精子。我们的数据表明，Sprm - 1 蛋白在单倍体精子细胞中发挥离散的调节功能，这是雄性生殖细胞最佳功能所必需的，但不是终末分化所必需的。
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