一种可能参与植物细胞壁合成的可逆糖基化多肽（RGP1）：纯化、基因克隆及反式高尔基体定位
A reversibly glycosylated polypeptide (RGP1) possibly involved in plant cell wall synthesis: purification, gene cloning, and trans-Golgi localization.
作者信息
Dhugga K S, Tiwari S C, Ray P M
机构信息
Department of Biological Sciences, Stanford University, Stanford, CA 94305, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7679-84. doi: 10.1073/pnas.94.14.7679.
Abstract
We purified from pea (Pisum sativum) tissue an approximately 40 kDa reversibly glycosylated polypeptide (RGP1) that can be glycosylated by UDP-Glc, UDP-Xyl, or UDP-Gal, and isolated a cDNA encoding it, apparently derived from a single-copy gene (Rgp1). Its predicted translation product has 364 aminoacyl residues and molecular mass of 41.5 kDa. RGP1 appears to be a membrane-peripheral protein. Immunogold labeling localizes it specifically to trans-Golgi dictyosomal cisternae. Along with other evidence, this suggests that RGP1 is involved in synthesis of xyloglucan and possibly other hemicelluloses. Corn (Zea mays) contains a biochemically similar and structurally homologous RGP1, which has been thought (it now seems mistakenly) to function in starch synthesis. The expressed sequence database also reveals close homologs of pea Rgp1 in Arabidopsis and rice (Oryza sativa). Rice possesses, in addition, a distinct but homologous sequence (Rgp2). RGP1 provides a polypeptide marker for Golgi membranes that should be useful in plant membrane studies.
摘要
我们从豌豆（Pisum sativum）组织中纯化出一种约40 kDa的可逆糖基化多肽（RGP1），它可被UDP-葡萄糖、UDP-木糖或UDP-半乳糖糖基化，并分离出编码该多肽的cDNA，该cDNA显然来源于单拷贝基因（Rgp1）。其预测的翻译产物有364个氨酰基残基，分子量为41.5 kDa。RGP1似乎是一种膜周边蛋白。免疫金标记将其特异性定位在反式高尔基体的潴泡上。结合其他证据，这表明RGP1参与木葡聚糖以及可能其他半纤维素的合成。玉米（Zea mays）含有一种生化性质相似且结构同源的RGP1，人们曾认为（现在看来是错误的）它在淀粉合成中起作用。表达序列数据库还揭示了拟南芥和水稻（Oryza sativa）中与豌豆Rgp1密切同源的序列。此外，水稻还拥有一个独特但同源的序列（Rgp2）。RGP1为高尔基体膜提供了一种多肽标记物，这在植物膜研究中应该会很有用。
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本文引用的文献
1
Functional compartmentation of the Golgi apparatus of plant cells : immunocytochemical analysis of high-pressure frozen- and freeze-substituted sycamore maple suspension culture cells.植物细胞高尔基体的功能区隔化：高压冷冻和冷冻替代的悬铃木悬浮培养细胞的免疫细胞化学分析。
Plant Physiol. 1992 Jul;99(3):1070-83. doi: 10.1104/pp.99.3.1070.
2
Auxin and Fusicoccin Enhancement of beta-Glucan Synthase in Peas : An Intracellular Enzyme Activity Apparently Modulated by Proton Extrusion.生长素和壳梭孢菌素对豌豆中β-葡聚糖合酶的增强作用：一种显然受质子外排调节的细胞内酶活性
Plant Physiol. 1985 Jul;78(3):466-72. doi: 10.1104/pp.78.3.466.
3
A Peanut Nodule Lectin in Infected Cells and in Vacuoles and the Extracellular Matrix of Nodule Parenchyma.一种存在于被感染细胞、液泡以及根瘤薄壁组织细胞外基质中的花生根瘤凝集素。
Plant Physiol. 1994 Feb;104(2):327-337. doi: 10.1104/pp.104.2.327.
4
Evidence for a UDP-Glucose Transporter in Golgi Apparatus-Derived Vesicles from Pea and Its Possible Role in Polysaccharide Biosynthesis.豌豆高尔基体衍生囊泡中UDP-葡萄糖转运蛋白的证据及其在多糖生物合成中的可能作用。
Plant Physiol. 1996 Dec;112(4):1585-1594. doi: 10.1104/pp.112.4.1585.
5
Higher plants contain homologs of the bacterial celA genes encoding the catalytic subunit of cellulose synthase.高等植物含有编码纤维素合酶催化亚基的细菌celA基因的同源物。
Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12637-42. doi: 10.1073/pnas.93.22.12637.
6
Identification of the major starch synthase in the soluble fraction of potato tubers.马铃薯块茎可溶性部分中主要淀粉合酶的鉴定。
Plant Cell. 1996 Jul;8(7):1121-35. doi: 10.1105/tpc.8.7.1121.
7
A guide to naming sequenced plant genes.已测序植物基因命名指南。
Plant Mol Biol. 1996 Jan;30(2):225-7. doi: 10.1007/BF00020109.
8
beta-Glucosylarginine: a new glucose-protein bond in a self-glucosylating protein from sweet corn.
FEBS Lett. 1995 Nov 27;376(1-2):61-4. doi: 10.1016/0014-5793(95)01247-6.
9
Effect of monensin on plant Golgi: re-examination of the monensin-induced changes in cisternal architecture and functional activities of the Golgi apparatus of sycamore suspension-cultured cells.莫能菌素对植物高尔基体的影响：对莫能菌素诱导的悬铃木悬浮培养细胞高尔基体的潴泡结构和功能活性变化的重新审视。
J Cell Sci. 1993 Mar;104 ( Pt 3):819-31. doi: 10.1242/jcs.104.3.819.
10
Effect of brefeldin A on the structure of the Golgi apparatus and on the synthesis and secretion of proteins and polysaccharides in sycamore maple (Acer pseudoplatanus) suspension-cultured cells.布雷菲德菌素A对梧桐（槭树）悬浮培养细胞中高尔基体结构以及蛋白质和多糖合成与分泌的影响
Plant Physiol. 1993 Apr;101(4):1363-73. doi: 10.1104/pp.101.4.1363.
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