Abkowitz J L, Brown K E, Wood R W, Kovach N L, Green S W, Young N S
Department of Medicine, University of Washington, Seattle 98195-7710, USA.
J Infect Dis. 1997 Jul;176(1):269-73. doi: 10.1086/517264.
Parvovirus B19 (B19) DNA was detected by dot blot hybridization in sera from 5 (17%) of 30 human immunodeficiency virus (HIV)-infected patients with hematocrits (HCT) of < or =24 and 4 (31%) of 13 HRV-infected patients with HCT of < or =20, suggesting that B19 is a reasonably common cause of severe anemia in HIV infection. The anemia promptly remitted after immunoglobulin therapy in 3 of 4 treated patients. The presence of IgM to B19, the clinical circumstance in which anemia developed, and the marrow morphology were poor predictors of chronic B19 infection. DNA hybridization studies of sera from 191 HIV-infected and 117 HIV-seronegative homosexual males attending a clinic in the Seattle area revealed that 1 (0.5%) and 2 (2%) samples, respectively, from the 2 groups contained B19. However, when assayed by polymerase chain reaction (PCR), 5% of the serum samples from HIV-infected persons and 9% from uninfected persons contained B19, although each had an HCT of > or =40. The data argue that anemia results from chronic high-titer B19 infection. Although a negative PCR assay excludes this diagnosis, DNA hybridization may be the more specific serum test.
通过斑点杂交法在30例血细胞比容（HCT）≤24的人类免疫缺陷病毒（HIV）感染患者中的5例（17%）血清以及13例HCT≤20的HIV感染患者中的4例（31%）血清中检测到细小病毒B19（B19）DNA，这表明B19是HIV感染中严重贫血的一个相当常见的病因。4例接受治疗的患者中有3例在免疫球蛋白治疗后贫血迅速缓解。针对B19的IgM的存在、贫血发生时的临床情况以及骨髓形态对慢性B19感染的预测性较差。对西雅图地区一家诊所的191例HIV感染和117例HIV血清阴性的同性恋男性的血清进行DNA杂交研究发现，两组分别有1份（0.5%）和2份（2%）样本含有B19。然而，当通过聚合酶链反应（PCR）检测时，HIV感染人群中有5%的血清样本以及未感染人群中有9%的血清样本含有B19，尽管他们的HCT均≥40。这些数据表明贫血是由慢性高滴度B19感染引起的。虽然PCR检测结果为阴性可排除该诊断，但DNA杂交可能是更具特异性的血清检测方法。
