Nouri Majid, Kamakifar Parvin, Khodabandehlou Niloofar, Sadri Nahand Javid, Tavakoli Ahmad, Norooznezhad Fatemeh, Sorayyayi Saba, Babaei Farhad, Mostafaei Shayan, Moghoofei Mohsen
Golestan Hospital Research Center, Tehran, Iran.
Department of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Med J Islam Repub Iran. 2019 Dec 16;33:137. doi: 10.34171/mjiri.33.137. eCollection 2019.
Human parvovirus B19 (B19V) can cause anemia in some patients, including those with compromised immunity system. There are a few studies on molecular epidemiology of B19V and its association with anemia in Iran. Therefore, the aim of this study was to determine the B19V DNA, IgM, IgG, genotyping, and viral load in HIV patients in different groups of pregnant women, general population, injection drug users (IDU), and Elite controllers. Also, the possible association of B19V with anemia was studied. In this case-control study, B19V DNA, anti-B19V IgM, anti-B19V IgG, viral load, and hemoglobin level were assessed in 113 HIV positive patients and 72 healthy controls. Also, CD4+ T cell counts and HIV load were measured in the patients' group. All statistical analyses were done using STATA 14.2 software (Stata Corporation, College Station, Texas, USA). P value < 0.05 was considered statistically significant. Among HIV patients, 19 (16.8%) cases had B19V DNA, 3 (2.7%) had B19V IgM, and 7 (6.2%) had B19V IgG. In control group, the prevalence of B19V DNA, IgM, and IgG was 6 (8.33%), 7(9.7%), and 19 (26.4%), respectively. In subpopulations based on transmission routes, general population had the highest B19V IgG and DNA positivity prevalence and viral load level. There was no significant association between B19V antibodies and DNA with anemia. The results demonstrated that B19V infection cannot be considered as a high-risk factor for anemia in adult HIV patients. However, further studies are needed to determine the exact role of B19V infection in HIV patients.
人细小病毒B19（B19V）可导致部分患者贫血，包括免疫系统受损者。伊朗有一些关于B19V分子流行病学及其与贫血关联的研究。因此，本研究旨在确定不同组别的孕妇、普通人群、注射吸毒者（IDU）和精英控制者中HIV患者的B19V DNA、IgM、IgG、基因分型和病毒载量。此外，还研究了B19V与贫血的可能关联。在这项病例对照研究中，对113例HIV阳性患者和72例健康对照者进行了B19V DNA、抗B19V IgM、抗B19V IgG、病毒载量和血红蛋白水平评估。同时，对患者组进行了CD4 + T细胞计数和HIV载量测量。所有统计分析均使用STATA 14.2软件（美国德克萨斯州大学站的Stata公司）完成。P值<0.05被认为具有统计学意义。在HIV患者中，19例（16.8%）检测出B19V DNA，3例（2.7%）检测出B19V IgM，7例（6.2%）检测出B19V IgG。对照组中，B19V DNA、IgM和IgG的患病率分别为6例（8.33%）、7例（9.7%）和19例（26.4%）。在基于传播途径的亚组中，普通人群的B19V IgG和DNA阳性患病率及病毒载量水平最高。B19V抗体和DNA与贫血之间无显著关联。结果表明，B19V感染不能被视为成年HIV患者贫血的高危因素。然而，需要进一步研究以确定B19V感染在HIV患者中的确切作用。
