Shapira L, Schatzker Y, Gedalia I, Borinski R, Sela M N
Department of Periodontology, Hebrew University-Hadassah Faculty of Dental Medicine, Jerusalem, Israel.
J Dent Res. 1997 Jul;76(7):1381-6. doi: 10.1177/00220345970760070801.
Amine fluoride (AmF)- and stannous fluoride (SnF2)-containing products were found to have a therapeutic effect on gingivitis and periodontitis. This effect was suggested to correlate with the antibacterial activity of the fluoride compounds. However, their effect on inflammatory cell function can also play a role in the therapeutic effect on gingival inflammation. The present study was designed to test the effects of AmF, SnF2, and an AmF/SnF2 combination on the function of human peripheral blood neutrophils, as compared with effects of chlorhexidine and salicylic acid. Neutrophils were isolated from human blood by ficoll centrifugation followed by dextran sedimentation. The neutrophils were pre-incubated with AmF, SnF2, or AmF/SnF2, followed by stimulation with fMLP. Cell vitality was verified by trypan-blue exclusion (> 95% vitality at all tested concentrations). Superoxide production was measured by cytochrome C reduction and the enzymatic activity of lysozyme and beta-glucoronidase by optical density measurement of substrate conversion. The results showed that AmF, SnF2, or AmF/SnF2 enhanced by two- to three-fold the superoxide release from fMLP-stimulated human neutrophils. Furthermore, the effective concentration of the AmF/SnF2 combination was several-fold lower than that of AmF or SnF2 alone (10 nM for AmF, 0.5 microM for SnF2, and 3 pM for SnF2/AmF). On the other hand, chlorhexidine and salicylic acid were found to reduce superoxide production by the cells. All the tested compounds had no effect on granular enzyme release by the stimulated neutrophils. The results suggest that AmF and SnF2 enhance the oxygen-dependent antibacterial activity of neutrophils. This effect may contribute to a more efficient elimination of bacteria from the periodontal environment, resulting in improvement in gingival health.
含胺氟化物（AmF）和氟化亚锡（SnF2）的产品被发现对牙龈炎和牙周炎有治疗作用。有人认为这种作用与氟化物化合物的抗菌活性相关。然而，它们对炎症细胞功能的影响也可能在对牙龈炎症的治疗作用中发挥作用。本研究旨在测试AmF、SnF2以及AmF/SnF2组合对人外周血中性粒细胞功能的影响，并与洗必泰和水杨酸的作用进行比较。通过菲可离心法随后进行葡聚糖沉淀从人血中分离中性粒细胞。中性粒细胞先用AmF、SnF2或AmF/SnF2预孵育，然后用fMLP刺激。通过台盼蓝排斥法验证细胞活力（在所有测试浓度下活力均>95%）。通过细胞色素C还原法测量超氧化物产生，通过底物转化的光密度测量法测量溶菌酶和β-葡萄糖醛酸酶的酶活性。结果表明，AmF、SnF2或AmF/SnF2使fMLP刺激的人中性粒细胞的超氧化物释放增加了两到三倍。此外，AmF/SnF2组合的有效浓度比单独的AmF或SnF2低几倍（AmF为10 nM，SnF2为0.5 μM，SnF2/AmF为3 pM）。另一方面，发现洗必泰和水杨酸会降低细胞的超氧化物产生。所有测试化合物对受刺激的中性粒细胞的颗粒酶释放均无影响。结果表明，AmF和SnF2增强了中性粒细胞的氧依赖性抗菌活性。这种作用可能有助于更有效地从牙周环境中清除细菌，从而改善牙龈健康。
