Beertsen W, Everts V
Anat Rec. 1977 Nov;189(3):479-97. doi: 10.1002/ar.1091890308.
In an attempt to localize the site of remodelling of collagen in the periodontal ligament of the continuously erupting mouse incisor a radioautographic and stereologic investigation was undertaken. Grain distributions in radioautographs for light microscopy were studied at various time intervals after administration of [3H]-proline. The distribution of rough endoplasmic reticulum cisternae in fibroblasts and the incidence of collagen phagocytosis across the ligament were studied by means of stereologic methods at the electron microscopic level. No significant differences were found in half lives of [3H]-labelled substances among the various regions across the ligament. Rough endoplasmic reticulum cisternae within fibroblasts were distributed more or less uniformly throughout the entire width of the ligament. Analysis of the distribution of collagen phagocytosis, however, revealed that in the midregion of the ligament the amount of phagocytosed collagen was approximately four times as high as in the area adjacent to the tooth and about nine times as high as in the alveolar compartment. It is concluded that synthesis and turnover of total protein occurs throughout the periodontal ligament but that remodelling of collagen predominantly takes place in an intermediate area of the ligament.
为了确定连续萌出的小鼠切牙牙周膜中胶原蛋白重塑的部位，进行了一项放射自显影和体视学研究。在给予[3H]-脯氨酸后的不同时间间隔，研究了光学显微镜放射自显影片中的颗粒分布。通过电子显微镜水平的体视学方法，研究了成纤维细胞中粗面内质网池的分布以及整个韧带中胶原吞噬的发生率。在整个韧带的不同区域，[3H]标记物质的半衰期没有发现显著差异。成纤维细胞内的粗面内质网池在韧带的整个宽度上分布或多或少均匀。然而，对胶原吞噬分布的分析表明，在韧带中部区域，吞噬的胶原量大约是邻近牙齿区域的四倍，是牙槽区的九倍左右。得出的结论是，整个牙周膜都发生总蛋白的合成和周转，但胶原的重塑主要发生在韧带的中间区域。
