日本青霉素耐药19B型肺炎链球菌菌株的遗传关系。
Genetic relationship of penicillin resistant Streptococcus pneumoniae serotype 19B strains in Japan.
作者信息
Yoshida R, Hirakata Y, Kaku M, Takemura H, Tanaka H, Tomono K, Koga H, Kohno S, Kamihira S
机构信息
Department of Laboratory Medicine, Nagasaki University School of Medicine, Japan.
出版信息
Epidemiol Infect. 1997 Apr;118(2):105-10. doi: 10.1017/s0950268896007273.
Pulsed field gel electrophoresis (PFGE) of the genomic DNA of penicillin resistant serotype 19B Streptococcus pneumoniae was carried out. Thirteen strains form the Nagasaki area and 12 strains from other areas in Japan were examined. Twenty-three strains were resistant to erythromycin, tetracycline and trimethoprim/sulfamethoxazole but susceptible to chloramphenicol. Eight strains were resistant to ceftriaxone. All strains were multiply resistant. Five strains isolated from Nagasaki were indistinguishable from each other by using restriction enzymes Apa I and Sma I. Two strains isolated from other areas were indistinguishable from the above five strains. We could classify 13 Nagasaki strains into 3 groups and the total of 25 Japanese strains into 6 groups. These results suggest that the increasing prevalence of multiply drug resistant S. pneumoniae serotyped 19B in Japan is not due to a single clone, but at least one clone has spread widely in Japan.
对青霉素耐药的19B型肺炎链球菌的基因组DNA进行了脉冲场凝胶电泳（PFGE）分析。检测了来自长崎地区的13株菌株和来自日本其他地区的12株菌株。23株菌株对红霉素、四环素和甲氧苄啶/磺胺甲恶唑耐药，但对氯霉素敏感。8株菌株对头孢曲松耐药。所有菌株均为多重耐药。使用限制性内切酶Apa I和Sma I时，从长崎分离出的5株菌株彼此无法区分。从其他地区分离出的2株菌株与上述5株菌株无法区分。我们可以将13株长崎菌株分为3组，将25株日本菌株总共分为6组。这些结果表明，日本多重耐药的19B型肺炎链球菌患病率的上升并非源于单一克隆，而是至少有一个克隆在日本广泛传播。
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