扫描激光多普勒血流仪测量视网膜和视神经乳头血流的可重复性。
Reproducibility of retinal and optic nerve head blood flow measurements with scanning laser Doppler flowmetry.
作者信息
Nicolela M T, Hnik P, Schulzer M, Drance S M
机构信息
Department of Ophthalmology, University of British Columbia, Vancouver, Canada.
出版信息
J Glaucoma. 1997 Jun;6(3):157-64.
PURPOSE
To evaluate the interobserver variability and the reproducibility of retinal and optic nerve head capillary blood flow measurements performed with a new noninvasive equipment, the scanning laser Doppler flowmeter (Heidelberg Engineering, Heidelberg, Germany).
METHODS
Blood flow measurements were performed during three independent sessions in six patients with glaucoma and five normal subjects using the scanning laser Doppler flowmeter (SLDF), which allows the visualization of perfused capillaries and vessels of the retina and optic nerve head and enables the quantification of capillary blood volume, flow, and velocity in any selected area of the perfusion map. To evaluate the interobserver variability in selecting the areas in the perfusion map to be measured, three observers tried to locate the same areas in the perfusion map of images obtained during the first session. To evaluate the reproducibility of the measurements, the observers measured correspondent areas in the peripapillary retina and in the optic nerve head of images from the three sessions. Areas of different sizes (10 x 10 pixels and 4 x 4 pixels) were measured.
RESULTS
The agreement between readings performed by the three observers was very good, with the reliability coefficient for the various parameters varying from 0.90 to 0.98. The reproducibility of retinal and lamina cribrosa measurements with the 10 x 10 pixel square target was good (reliability coefficient for the different parameters ranging from 0.70 to 0.85) and much better than the reproducibility of the 4 x 4 pixel target. The measurements performed in the neuroretinal rim area also had poor reproducibility. The measurements from the patients with glaucoma tended to be more reproducible than those from normal subjects.
CONCLUSIONS
The SLDF allows reproducible blood perfusion measurements of retinal and lamina cribrosa areas when a target square of 10 x 10 pixels is used.
目的
使用一种新型非侵入性设备——扫描激光多普勒血流仪（德国海德堡海德堡工程公司），评估视网膜和视神经乳头毛细血管血流测量的观察者间变异性和可重复性。
方法
使用扫描激光多普勒血流仪（SLDF），对6例青光眼患者和5名正常受试者在三个独立时间段进行血流测量。该仪器能够可视化视网膜和视神经乳头的灌注毛细血管和血管，并能对灌注图中任何选定区域的毛细血管血容量、血流和速度进行量化。为评估观察者在灌注图中选择测量区域时的观察者间变异性，三名观察者试图在第一次测量期间获得的图像灌注图中定位相同区域。为评估测量的可重复性，观察者对三个时间段图像的视乳头周围视网膜和视神经乳头中的相应区域进行测量。测量了不同大小（10×10像素和4×4像素）的区域。
结果
三名观察者的读数之间一致性非常好，各种参数的可靠性系数在0.90至0.98之间。使用10×10像素方形目标时，视网膜和筛板测量的可重复性良好（不同参数的可靠性系数在0.70至0.85之间），且远优于4×4像素目标的可重复性。在神经视网膜边缘区域进行的测量可重复性也较差。青光眼患者的测量结果往往比正常受试者的测量结果更具可重复性。
结论
当使用10×10像素的方形目标时，SLDF能够对视网膜和筛板区域进行可重复的血流灌注测量。
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