Mifsud A J, Watine J, Picard B, Charet J C, Solignac-Bourrel C, Pitt T L
Laboratory of Hospital Infection, Central Public Health Laboratory, Colindale, London, UK.
J Hosp Infect. 1997 Jun;36(2):105-16. doi: 10.1016/s0195-6701(97)90116-x.
A clonal origin for European isolates of antibiotic multi-resistant Pseudomonas aeruginosa serotype O12 has been suggested. This study was designed to assess the value and limitations of several typing methods for the investigation of outbreaks due to this serotype. In Hôpital de Rodez, France, this organism is endemic, and a prospective clinical epidemiological study was undertaken over a 15 month period, encompassing all patients at the hospital from whom P. aeruginosa O12 was isolated. All isolates were examined by auxanogram, antibiogram, phage-typing, electrophoresis of esterases and pulsed-field gel electrophoresis of DNA. The results suggest that (1) the methods used did not clearly differentiate between clinically-related and epidemiologically-unrelated European isolates, (2) in Hôpital de Rodez, while some isolates were likely to have been transmitted from patient-to-patient, most infections or colonizations with this organism were sporadic and their origin is unknown. The limits of typing methods for the investigation of outbreaks of nosocomial infection with multi-resistant P. aeruginosa O12 are emphasized.
有人提出，欧洲分离出的抗生素多重耐药性O12血清型铜绿假单胞菌具有克隆起源。本研究旨在评估几种分型方法在调查该血清型引起的疫情中的价值和局限性。在法国罗德兹医院，这种病菌呈地方性流行，我们进行了一项为期15个月的前瞻性临床流行病学研究，涵盖了该医院所有分离出O12血清型铜绿假单胞菌的患者。所有分离株均通过生长谱测定、抗菌谱分析、噬菌体分型、酯酶电泳和DNA脉冲场凝胶电泳进行检测。结果表明：（1）所使用的方法未能明确区分临床相关和流行病学无关的欧洲分离株；（2）在罗德兹医院，虽然一些分离株可能在患者之间传播，但该病菌的大多数感染或定植是散发性的，其来源不明。文中强调了用于调查多重耐药性O12血清型铜绿假单胞菌医院感染暴发的分型方法的局限性。
