分子流行病学为一家儿科医院中多重耐药性O:12血清型铜绿假单胞菌暴发分离株的基因型异质性提供了证据。
Molecular epidemiology provides evidence of genotypic heterogeneity of multidrug-resistant Pseudomonas aeruginosa serotype O:12 outbreak isolates from a pediatric hospital.
作者信息
Bingen E, Bonacorsi S, Rohrlich P, Duval M, Lhopital S, Brahimi N, Vilmer E, Goering R V
机构信息
Service de Microbiologie, Hôpital Robert Debré, Paris, France.
出版信息
J Clin Microbiol. 1996 Dec;34(12):3226-9. doi: 10.1128/jcm.34.12.3226-3229.1996.
Abstract
Ribotyping randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis were used for the epidemiologic evaluation of eight Pseudomonas aeruginosa O:12 isolates obtained from eight children and two P. aeruginosa O:12 environmental isolates from a hematology ward. Randomly amplified polymorphic DNA analysis and pulsed-field gel electrophoresis were able to discriminate isolates that were indistinguishable by biochemical typing, O serotyping or ribotyping.
摘要
核糖体分型、随机扩增多态性DNA分析和脉冲场凝胶电泳用于对从8名儿童分离得到的8株铜绿假单胞菌O:12菌株以及从一个血液科病房分离得到的2株铜绿假单胞菌O:12环境菌株进行流行病学评估。随机扩增多态性DNA分析和脉冲场凝胶电泳能够区分那些通过生化分型、O血清型分型或核糖体分型无法区分的菌株。
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