Laria C, Alió J L, Ruiz-Moreno J M
Ophthalmology Department, University of Alicante, Spain.
Ophthalmic Res. 1997;29(3):145-53. doi: 10.1159/000268009.
The effects of anti-inflammatory non-steroidal therapy combined with free-radical scavengers were studied and compared to corticosteroid use in the treatment of experimental corneal injury.
Eighty New Zealand albino rabbits were used in this study. A corneal alkali burn was induced by applying 1-N NaOH filter paper on the central axis of the right cornea for 30 s. Animals were distributed into five treatment groups: group 1 (control group) was only given gentamicin; group 2 was treated with 0.5% dimethylthiourea (DMU); group 3 received 1% dexamethasone; group 4 was given combined 0.5% DMU and 1% indomethacin; group 5 was treated with 0.5% DMU and 0.1% diclofenac sodium. One 50-microliter drop of gentamicin was instilled every 12 h, whereas the other drugs were instilled every 6 h (50 microliters). All groups received the same antibiotic treatment as the control group. The animals were killed on the 5th day. Inflammatory index, area and perimeter of the wounded corneal zone, and corneal transparency were evaluated.
No significant differences in the inflammatory index were found between the treatment groups and the control group after 72 h. Significant differences (p < 0.001) were observed at 24 h in groups 3-5 when compared with the control group. Planimetry showed significant differences in group 4 when compared with the other groups (p < 0.05). Corneal transparency study showed statistically significantly better values in groups 4 and 5, when compared with the other groups, including group 3 (p < 0.05).
The use of 0.5% DMU combined with 1% indomethacin can be considered an alternative to corticosteroid treatment in our experimental chemical corneal injury.
研究抗炎非甾体疗法联合自由基清除剂的效果，并与皮质类固醇用于治疗实验性角膜损伤的效果进行比较。
本研究使用了80只新西兰白化兔。通过将1-N氢氧化钠滤纸敷于右眼角膜中轴线30秒诱导角膜碱烧伤。动物被分为五个治疗组：第1组（对照组）仅给予庆大霉素；第2组用0.5%二甲硫脲（DMU）治疗；第3组接受1%地塞米松治疗；第4组给予0.5% DMU和1%吲哚美辛联合治疗；第5组用0.5% DMU和0.1%双氯芬酸钠治疗。每12小时滴注一滴50微升庆大霉素，而其他药物每6小时滴注一次（50微升）。所有组接受与对照组相同的抗生素治疗。在第5天处死动物。评估炎症指数、受伤角膜区域的面积和周长以及角膜透明度。
72小时后，治疗组与对照组之间的炎症指数无显著差异。与对照组相比，第3 - 5组在24小时时观察到显著差异（p < 0.001）。面积测量显示第4组与其他组相比有显著差异（p < 0.05）。角膜透明度研究显示，与包括第3组在内的其他组相比，第4组和第5组在统计学上有显著更好的值（p < 0.05）。
在我们的实验性化学性角膜损伤中，使用0.5% DMU联合1%吲哚美辛可被视为皮质类固醇治疗的替代方法。
