Smetana K, Lukás J, Palecková V, Bartůnkovä J, Liu F T, Vacík J, Gabius H J
Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic.
Biomaterials. 1997 Jul;18(14):1009-14. doi: 10.1016/s0142-9612(97)00037-9.
The reactivity of diverse immune aspects to the presence of synthetic polymers represents one of the most important aspects of implantable device biocompatibility. In this report, we show the effect of the chemical structure of a synthetic polymer support on monocyte adhesion and selected phenotypic characteristics in vitro as a model for the initial steps of non-self-recognition of an implant. The extent of monocyte adhesion was significantly influenced by the support chemistry. The highest level of monocyte adhesion was observed on a surface copolymer of 2-hydroxyethyl methacrylate with dimethyl aminoethyl methacrylate relative to results of experiments in which poly(2-hydroxyethyl methacrylate) or the copolymer of 2-hydroxyethyl methacrylate with the sodium salt of methacrylic acid was used. Cell adhesion to the polymers tested and to glass was accompanied by enhanced expression of the carbohydrate-binding sites tested for asialoglycoprotein beta-galactosides such as galectins, beta-N-acetylgalactosamine, alpha-mannoside, specific lectin for heparin as well as the lymphokine-macrophage migration inhibitory factor in the monocytes tested. These results suggest the importance of monocyte adhesion to the biomaterial surface for their development into macrophages and further non-self-recognition of the implanted device.
多种免疫反应对合成聚合物存在的反应性是可植入设备生物相容性最重要的方面之一。在本报告中，我们展示了合成聚合物载体的化学结构对单核细胞黏附以及体外选定表型特征的影响，以此作为植入物非自我识别初始步骤的模型。单核细胞黏附的程度受到载体化学性质的显著影响。相对于使用聚（甲基丙烯酸2-羟乙酯）或甲基丙烯酸2-羟乙酯与甲基丙烯酸钠盐的共聚物进行的实验结果，在甲基丙烯酸2-羟乙酯与甲基丙烯酸二甲氨基乙酯的表面共聚物上观察到了最高水平的单核细胞黏附。测试的聚合物以及玻璃上的细胞黏附伴随着测试的单核细胞中针对去唾液酸糖蛋白β-半乳糖苷（如半乳糖凝集素）、β-N-乙酰半乳糖胺、α-甘露糖苷、肝素特异性凝集素以及淋巴因子-巨噬细胞迁移抑制因子的碳水化合物结合位点表达增强。这些结果表明单核细胞黏附于生物材料表面对于其发育为巨噬细胞以及对植入设备的进一步非自我识别具有重要意义。
