通过免疫金电子显微镜检测和比较分析克罗恩病中持续性麻疹病毒感染情况。

Detection and comparative analysis of persistent measles virus infection in Crohn's disease by immunogold electron microscopy.

作者信息

Daszak P, Purcell M, Lewin J, Dhillon A P, Pounder R E, Wakefield A J

机构信息

Inflammatory Bowel Disease Study Group, School of Life Sciences, Kingston University, Kingston-on-Thames, Surrey, United Kingdom.

出版信息

J Clin Pathol. 1997 Apr;50(4):299-304. doi: 10.1136/jcp.50.4.299.

DOI:10.1136/jcp.50.4.299

PMID:9215145

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC499879/

Abstract

AIMS

To determine the specificity of persistent measles virus infection in intestinal samples from Crohn's disease patients using quantitative immunogold electron microscopy. To compare the results with samples from ulcerative colitis, a granulomatous inflammatory control (tuberculous lymphadenitis), and a positive control.

METHODS

Formalin fixed, paraffin embedded intestinal tissue from patients with Crohn's disease was reprocessed and stained with antimeasles nucleocaspid protein primary antibody followed by 10 nm gold conjugated secondary antibody. Tissue samples were taken from granulomatous and non-granulomatous areas of the intestine. Intestinal samples from patients with ulcerative colitis, tuberculous lymphadenitis, or acute mesenteric ischaemia were similarly processed. Brain tissue from a patient with subacute sclerosing panencephalitis (SSPE) was used as the positive control. Duplicate sections of all tissues were processed without the primary antibody. Stained specimens were examined by electron microscopy.

RESULTS

In Crohn's disease patients, 8/9 foci of granulomatous inflammation and 0/4 foci of non-specific inflammation were positive for measles virus. Of controls, 0/5 non-inflamed intestinal tissues, 1/8 tuberculous tissues, 1/5 ulcerative colitis tissues, and 1/1 SSPE tissues were positive. Gold grain counts per nuclear field-of-view in both Crohn's disease granulomas (43.29) and SSPE (36.94) were significantly higher than in tissues from patients with ulcerative colitis (13.52) or tuberculous lymphadenitis (15.875), and nongranulomatous areas of Crohn's disease (4.89) (p < 0.001, p < 0.001, p = 0.0006, respectively), with no significant difference between Crohn's disease and SSPE (p > 0.1). In both SSPE and Crohn's disease staining was confined to a small population of cells exhibiting characteristic cytopathology.

CONCLUSION

These data support a role for measles virus in the aetiology of Crohn's disease.

摘要

目的

运用定量免疫金电子显微镜技术确定克罗恩病患者肠道样本中持续性麻疹病毒感染的特异性。并将结果与溃疡性结肠炎、肉芽肿性炎症对照（结核性淋巴结炎）及阳性对照的样本进行比较。

方法

对克罗恩病患者经福尔马林固定、石蜡包埋的肠道组织进行重新处理，先用抗麻疹核衣壳蛋白一抗染色，再用10纳米金标记二抗染色。组织样本取自肠道的肉芽肿性和非肉芽肿性区域。溃疡性结肠炎、结核性淋巴结炎或急性肠系膜缺血患者的肠道样本也进行类似处理。亚急性硬化性全脑炎（SSPE）患者的脑组织用作阳性对照。所有组织的复切片在不使用一抗的情况下进行处理。染色标本通过电子显微镜检查。

结果

在克罗恩病患者中，8/9个肉芽肿性炎症病灶和0/4个非特异性炎症病灶麻疹病毒呈阳性。在对照中，0/5个非炎症性肠道组织、1/8个结核组织、1/5个溃疡性结肠炎组织和1/1个SSPE组织呈阳性。克罗恩病肉芽肿（43.29）和SSPE（36.94）中每个核视野的金颗粒计数均显著高于溃疡性结肠炎患者组织（13.52）或结核性淋巴结炎患者组织（15.875）以及克罗恩病非肉芽肿性区域（4.89）（p分别<0.001、<0.001、=0.0006），克罗恩病与SSPE之间无显著差异（p>0.1）。在SSPE和克罗恩病中，染色均局限于一小部分呈现特征性细胞病理学的细胞。