Frönicke L, Müller-Navia J, Romanakis K, Scherthan H
Abteilung für Humanbiologie und Humangenetik, Universität Kaiserslautern, Postfach 3049, D-67653 Kaiserslautern, Germany.
Chromosoma. 1997 Jul;106(2):108-13. doi: 10.1007/s004120050230.
We report on the construction of the first comparative Zoo-FISH map of a marine mammal. Zoo-FISH with DNA probes from a human chromosome-specific library to metaphase spreads of the harbor seal (Phoca vitulina) disclosed 31 conserved syntenic segments covering the complete autosomal complement and the X chromosome. Comparison with Zoo-FISH maps of other species reveals that the harbor seal shares a high degree of karyotypic homeology with the human complement and an even higher degree with the conordinal cat complement. These findings suggest that pinniped, felid and human karyotypes have maintained conserved complements. Based on data of Zoo-FISH and comparative cytogenetics, a Zoo-FISH map of the ancestral carnivore karyotype (Z-CAR) is proposed. Flow cytometry revealed that the DNA value of the harbor seal genome is 79% that of the human genome.
我们报告了首个海洋哺乳动物比较荧光原位杂交（Zoo-FISH）图谱的构建情况。用人染色体特异性文库的DNA探针与港海豹（Phoca vitulina）中期染色体铺片进行Zoo-FISH分析，发现了31个保守的同线区段，覆盖了整个常染色体组和X染色体。与其他物种的Zoo-FISH图谱比较表明，港海豹与人类染色体组具有高度的核型同源性，与同目猫科动物的染色体组同源性更高。这些发现表明，鳍足类动物、猫科动物和人类的核型保持了保守的染色体组。基于Zoo-FISH和比较细胞遗传学数据，提出了一个祖先食肉动物核型的Zoo-FISH图谱（Z-CAR）。流式细胞术显示，港海豹基因组的DNA值是人类基因组的79%。
