Kuo S M, Morehouse H F, Lin C P
Nutrition Program, State University of New York at Buffalo, 14214, USA.
Cancer Lett. 1997 Jun 24;116(2):131-7. doi: 10.1016/s0304-3835(97)00183-3.
Dietary flavonoids were found to be antiproliferative for human colon cancer cells, Caco-2 and HT-29, and rat nontransformed intestinal crypt cells, IEC-6. The antiproliferative potency was found to be structure-dependent. We report here a correlation between the antiproliferative potency of these flavonoids and their ability to inhibit cellular accumulation of ascorbic acid (vitamin C). Caco-2, HT-29 and IEC-6 cells were found to accumulate ascorbic acid in a sodium-dependent fashion although some ascorbic acid may also enter the cells through sodium-independent mechanisms. Flavonoids that have been found to be antiproliferative, quercetin and genistein, inhibited the accumulation of ascorbic acid. The inhibition was dose-dependent and could be observed after as short as 10-min of incubation. The degree of inhibition of accumulation was more during rapid cell division as compared to post-confluency Caco-2 cells. Flavonoids that were found to show little antiproliferative effect, naringenin and catechin, also had little effect on ascorbic acid accumulation. The antiproliferative property of flavonoids could be linked to their ascorbic acid deprivation property.
膳食类黄酮被发现对人结肠癌细胞Caco - 2和HT - 29以及大鼠未转化的肠隐窝细胞IEC - 6具有抗增殖作用。发现其抗增殖能力与结构有关。我们在此报告这些类黄酮的抗增殖能力与其抑制细胞内抗坏血酸（维生素C）积累能力之间的相关性。尽管一些抗坏血酸也可能通过不依赖钠的机制进入细胞，但发现Caco - 2、HT - 29和IEC - 6细胞以依赖钠的方式积累抗坏血酸。已发现具有抗增殖作用的类黄酮槲皮素和染料木黄酮可抑制抗坏血酸的积累。这种抑制呈剂量依赖性，且在孵育仅10分钟后即可观察到。与汇合后的Caco - 2细胞相比，在细胞快速分裂期间积累的抑制程度更大。已发现几乎没有抗增殖作用的类黄酮柚皮素和儿茶素，对抗坏血酸的积累也几乎没有影响。类黄酮的抗增殖特性可能与其剥夺抗坏血酸的特性有关。
