Kuo S M
Nutrition Program, State University of New York at Buffalo, 14214, USA.
Cancer Lett. 1996 Dec 20;110(1-2):41-8. doi: 10.1016/s0304-3835(96)04458-8.
Dietary flavonoids are known to be antiproliferative and may play an important role in cancer chemoprevention, especially cancers of the gastrointestinal tract, because of a direct contact with food. This study was designed to compare the antiproliferative potency of several structurally distinct dietary flavonoids in colon cancer cells, Caco-2 and HT-29, and in rat non-transformed intestinal crypt cells, IEC-6. Flavonoids varied significantly in their antiproliferative potency depending on the structural features but the observations were consistent among the three cell lines studied. Of the two most potent flavonoids, quercetin and genistein, the effect was found to be dose-dependent and chromatin condensation, an indication of apoptosis, was noticed. Quercetin was found to distribute throughout the cell with higher amounts in the perinuclear and nucleoli areas. The lack of specific cell membrane enrichment by quercetin was consistent with its lack of effect on the transepithelial resistance. While several flavonoids including quercetin were found to be unstable, the chemical instability did not correlate with the antiproliferative potency, although it may contribute to the antiproliferative effect.
膳食类黄酮具有抗增殖作用，由于其与食物直接接触，可能在癌症化学预防中发挥重要作用，尤其是胃肠道癌症。本研究旨在比较几种结构不同的膳食类黄酮对结肠癌细胞Caco-2和HT-29以及大鼠未转化的肠隐窝细胞IEC-6的抗增殖能力。根据结构特征，类黄酮的抗增殖能力差异显著，但在所研究的三种细胞系中观察结果一致。在两种最有效的类黄酮槲皮素和染料木黄酮中，发现其作用呈剂量依赖性，并且观察到染色质浓缩，这是细胞凋亡的一个迹象。发现槲皮素分布于整个细胞，在核周和核仁区域含量较高。槲皮素缺乏对细胞膜的特异性富集，这与其对跨上皮电阻无影响一致。虽然包括槲皮素在内的几种类黄酮被发现不稳定，但其化学不稳定性与抗增殖能力无关，尽管它可能有助于抗增殖作用。
