Nielsen F, Mikkelsen B B, Nielsen J B, Andersen H R, Grandjean P
Institute of Community Health, Odense University, Denmark.
Clin Chem. 1997 Jul;43(7):1209-14.
Malondialdehyde (MDA) is one of the most frequently used indicators of lipid peroxidation. To generate reliable reference intervals for plasma malondialdehyde (P-MDA), a reference sample group was established in Funen, Denmark. The group consisted of 213 individuals (107 men, 106 women), ages 20-79 years. P-MDA was measured in EDTA-treated plasma after derivatization by thiobarbituric acid (TBA) and separation on HPLC. UV detection was performed at 532 nm. A reference interval was calculated as recommended by IFCC with REFVAL 3.42. The estimated reference limits (0.025 and 0.975 fractals) for the group were 0.36 and 1.24 mumol/L. The data were analyzed for gender- and age-related differences. Analysis of variance showed no interaction between gender and age, but separate analyses showed an independent effect of gender (P = 0.03), but not of age (P = 0.11). Daily smokers had a slightly higher average concentration of P-MDA than nonsmokers (P = 0.05), and P-MDA correlated with daily exposure to cigarette smoke (r = 0.162; P = 0.03). A positive correlation was also demonstrated between P-MDA and weekly alcohol consumption (r = 0.153; P = 0.03). Within-subject and day-to-day variations of P-MDA indicated that the potential of P-MDA as a biomarker for individuals is questionable. However, on a group basis, the present data support that P-MDA may be a potential biomarker for oxidative stress.
丙二醛（MDA）是脂质过氧化最常用的指标之一。为了生成可靠的血浆丙二醛（P-MDA）参考区间，在丹麦富宁建立了一个参考样本组。该组由213名个体组成（107名男性，106名女性），年龄在20至79岁之间。通过硫代巴比妥酸（TBA）衍生化并在高效液相色谱（HPLC）上分离后，在乙二胺四乙酸（EDTA）处理的血浆中测量P-MDA。在532nm处进行紫外检测。按照国际临床化学和检验医学联合会（IFCC）推荐并使用REFVAL 3.42计算参考区间。该组的估计参考限（第0.025和0.975分位数）为0.36和1.24μmol/L。对数据进行了性别和年龄相关差异分析。方差分析显示性别和年龄之间无交互作用，但单独分析显示性别有独立影响（P = 0.03），而年龄无独立影响（P = 0.11）。每日吸烟者的P-MDA平均浓度略高于非吸烟者（P = 0.05），且P-MDA与每日接触香烟烟雾相关（r = 0.162；P = 0.03）。P-MDA与每周酒精摄入量之间也呈正相关（r = 0.153；P = 0.03）。P-MDA的个体内和日间变化表明，P-MDA作为个体生物标志物的潜力值得怀疑。然而，基于群体而言，目前的数据支持P-MDA可能是氧化应激的潜在生物标志物。
