Chen M H, Benjamin T
Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Virology. 1997 Jul 7;233(2):440-2. doi: 10.1006/viro.1997.8596.
Specific glycosylation inhibitors were used to show that N-glycans, and not O-glycans or glycolipids, constitute the major class of receptors on 3T3 cells for polyoma virus. Sialic acid (SA)-specific lectins were used in attempts to confirm the expected SA linkage on N-linked glycans. A lectin specific for alpha2,6 linked SA, a non-receptor type, was slightly more effective in blocking infection than a lectin specific for alpha2,3 linked SA which corresponds to the known receptor specificity. Possible explanations are suggested for this unexpected result.
使用特定的糖基化抑制剂来表明，构成多瘤病毒在3T3细胞上主要受体类别的是N-聚糖，而非O-聚糖或糖脂。使用唾液酸（SA）特异性凝集素来尝试确认N-连接聚糖上预期的SA连接。一种对α2,6连接的SA特异的凝集素（一种非受体类型）在阻断感染方面比一种对α2,3连接的SA特异的凝集素更有效，后者对应于已知的受体特异性。针对这一意外结果提出了可能的解释。
