在患有各种皮肤淋巴增殖性疾病的HIV阴性患者中未发现人类疱疹病毒8型DNA序列的证据。

Lack of evidence of human herpesvirus 8 DNA sequences in HIV-negative patients with various lymphoproliferative disorders of the skin.

作者信息

Dupin N, Franck N, Calvez V, Gorin I, Grandadam M, Huraux J M, Leibowitch M, Agut H, Escande J P

机构信息

Department of Dermatology, Hôpital Tarnier-Cochin, France.

出版信息

Br J Dermatol. 1997 Jun;136(6):827-30. doi: 10.1111/j.1365-2133.1997.tb03920.x.

DOI:10.1111/j.1365-2133.1997.tb03920.x

PMID:9217812

Abstract

Human herpesvirus 8 (HHV-8) is a new virus which has been reported in Kaposi's sarcoma and some lymphoproliferative disorders such as Castleman's disease and body-cavity-based lymphoma. Because HHV-8 shares homology with Epstein-Barr virus (EBV), we searched for the presence of HHV-8 DNA sequences in various cutaneous T- and B-cell lymphoma by the polymerase chain reaction (PCR). Forty-seven HIV-negative patients with cutaneous lymphoma or large plaque parapsoriasis were enrolled in the study. For the detection of HHV-8 DNA sequences we used PCR followed by a hybridization with a digoxigenin-labelled probe and nested-PCR. HHV-8 DNA sequences could only be detected in a patient with large plaque parapsoriasis. Our study does not suggest any direct implication of HHV-8 in the pathogenesis of most cutaneous lymphoma. Serological studies will be helpful to appreciate if there is an epidemiological link between HHV-8 and cutaneous lymphomas.

摘要

人类疱疹病毒8型（HHV - 8）是一种新发现的病毒，已在卡波西肉瘤以及某些淋巴增殖性疾病（如Castleman病和体腔淋巴瘤）中被报道。由于HHV - 8与爱泼斯坦 - 巴尔病毒（EBV）具有同源性，我们通过聚合酶链反应（PCR）在各种皮肤T细胞和B细胞淋巴瘤中寻找HHV - 8 DNA序列的存在。47例HIV阴性的皮肤淋巴瘤或大斑块状副银屑病患者参与了该研究。为了检测HHV - 8 DNA序列，我们先使用PCR，然后与地高辛标记的探针进行杂交以及巢式PCR。HHV - 8 DNA序列仅在一名大斑块状副银屑病患者中被检测到。我们的研究并未表明HHV - 8在大多数皮肤淋巴瘤的发病机制中有任何直接关联。血清学研究将有助于了解HHV - 8与皮肤淋巴瘤之间是否存在流行病学联系。