Ben-Smith A, Goodall J C, Gaston J S, Winer J B
Centre for Clinical Research in Immunology and Signalling, Medical School, University of Birmingham, UK.
Clin Exp Immunol. 1997 Jul;109(1):121-6. doi: 10.1046/j.1365-2249.1997.4221318.x.
In three patients whose Guillain-Barré syndrome (GBS) was preceded by gastrointestinal infection due to Campylobacter jejuni, gammadelta T cells were generated from peripheral blood in response to in vitro stimulation with C. jejuni. In one of the patients, where a diagnostic sural nerve biopsy was performed, gammadelta T cells were also isolated following culture of the nerve tissue. Studies with healthy volunteers and C. jejuni gastroenteritis patients also showed preferential enrichment for gammadelta T cells in peripheral blood cells stimulated with C. jejuni, although the response was significantly lower than that seen in GBS patients. In two out of three GBS patients and all of the controls, gammadelta T cell receptor (TCR) gene usage was shown to be Vgamma9/Vdelta2+. In the GBS patient where nerve-infiltrating gammadelta T cells were isolated, these and C. jejuni-specific peripheral blood cells had similar TCR gene usage, predominantly consisting of Vgamma5/Vdelta1+ cells. Sequencing the Vdelta1 products from nerve and peripheral blood showed similarities in CDR3 length, but the single Vdelta1 sequence obtained from nerve was not identified in peripheral blood. These results suggest that the generation of gammadelta T cells is part of a normal immune response to C. jejuni, which, in patients with GBS, may contribute to the pathogenesis of their inflammatory neuropathy.
在3例吉兰-巴雷综合征（GBS）发病前有空肠弯曲菌引起的胃肠道感染的患者中，外周血中的γδT细胞在体外受到空肠弯曲菌刺激后产生。其中1例患者进行了诊断性腓肠神经活检，神经组织培养后也分离出了γδT细胞。对健康志愿者和空肠弯曲菌性肠胃炎患者的研究还表明，用空肠弯曲菌刺激外周血细胞后，γδT细胞会优先富集，尽管其反应明显低于GBS患者。在3例GBS患者中的2例以及所有对照组中，γδT细胞受体（TCR）基因使用情况显示为Vγ9/Vδ2+。在分离出神经浸润性γδT细胞的GBS患者中，这些细胞和空肠弯曲菌特异性外周血细胞具有相似的TCR基因使用情况，主要由Vγ5/Vδ1+细胞组成。对神经和外周血中的Vδ1产物进行测序显示，互补决定区3（CDR3）长度相似，但从神经中获得的单个Vδ1序列在外周血中未被识别。这些结果表明，γδT细胞的产生是对空肠弯曲菌正常免疫反应的一部分，在GBS患者中，这可能有助于其炎性神经病变的发病机制。
