Finette B A, Poseno T, Vacek P M, Albertini R J
Department of Pediatrics, University of Vermont, Burlington 05401, USA.
Mutat Res. 1997 Jun 9;377(1):115-23. doi: 10.1016/s0027-5107(97)00069-9.
We utilized the hprt T-cell cloning assay to prospectively determined the somatic mutant frequency at the hprt locus of fetal T-lymphocytes exposed in utero to maternal active and passive cigarette smoke. In addition, a maternal questionnaire was administered to evaluate a number of social and medical parameters that may effect hprt mutant frequency. Newborn cord blood plasma cotinine levels were determined on all subjects to compare in utero tobacco metabolite levels with maternal smoking histories. A total of 63 newborns were enrolled and placed into four groups: Group I (n = 21), newborns whose mothers had no history of active or passive cigarette exposure during the pregnancy; Group II (n = 12), newborns whose mothers actively smoked cigarettes throughout the pregnancy; Group III (n = 8), newborns whose mothers actively smoked cigarettes during first trimester only; and Group IV (n = 22), newborns whose mothers were exposed only to passive cigarette smoke. Our analysis showed no statistically significant difference in hprt mutation frequency between any of the four groups. A significant increase in plasma cord blood cotinine was detected in Group II, newborns whose mothers were active cigarette smokers throughout the pregnancy. Our data indicate that exposure to active and passive maternal cigarette smoke in utero does not result in a significant increase in somatic mutant frequency as determined by the hprt T-cell cloning assay.
我们利用次黄嘌呤磷酸核糖转移酶（hprt）T细胞克隆试验，前瞻性地测定了子宫内暴露于母亲主动和被动吸烟环境下的胎儿T淋巴细胞hprt位点的体细胞突变频率。此外，还进行了一项母亲问卷调查，以评估一些可能影响hprt突变频率的社会和医学参数。测定了所有受试者的新生儿脐带血血浆可替宁水平，以比较子宫内烟草代谢物水平与母亲吸烟史。总共招募了63名新生儿，并将其分为四组：第一组（n = 21），母亲在孕期无主动或被动吸烟史的新生儿；第二组（n = 12），母亲在整个孕期均主动吸烟的新生儿；第三组（n = 8），母亲仅在孕早期主动吸烟的新生儿；第四组（n = 22），母亲仅暴露于被动吸烟环境的新生儿。我们的分析显示，四组中的任何一组之间，hprt突变频率均无统计学上的显著差异。在第二组（母亲在整个孕期均为主动吸烟者的新生儿）中，检测到脐带血血浆可替宁显著增加。我们的数据表明，子宫内暴露于母亲主动和被动吸烟环境下，并不会导致次黄嘌呤磷酸核糖转移酶（hprt）T细胞克隆试验所测定的体细胞突变频率显著增加。
